The metastatic TS/A line has been recently derived from a spontaneous BALB/c mammary tumor. When TS/A cells were cultured in 0·33 per cent agar, two morphologically distinct types of colonies were observed from which two sets of clones were obtained. E clones were derived from small, transparent colonies, whereas F clones were from large, thick, actively growing colonies.
All the clones were tumorigenic in syngeneic BALB/c females. However, E clones showed higher ability than F clones to metastasize spontaneously to the lung. Comparison between E and F clones shows that the high level of spontaneous metastasization to the lung is associated with epithelial-likein vitro growth pattern, spontaneous dome formation and growth pattern in 0·33 per cent agar cultures. The ability to give rise to lung colonies following intravenous inoculation is not a predictive parameter for the spontaneous metastatic potential.
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References
Dexter, D. L., Kowalski, H. M., Blazar, B. A., Fcligiel, Z., Vogel, R., and Heppner, G., 1978, Heterogeneity of tumor cells from a single mouse mammary tumor.Cancer Research,38, 3174–3181.
Dulbecco, R., Henahan, M., Bowman, M., Okada, S., Battifora, H., and Unger, M., 1981, Generation of fibroblast-like cells from cloned epithelial mammary cellsin vitro: a possible new cell type.Proceedings of the National Academy of Science, USA,78, 2345–2349.
Fidler, I. J., and Kripke, M. L., 1977, Metastasis results from preexisting variant cells within a malignant tumor.Science,197, 893–895.
Hager, J. C., Russo, J., Ceriani, R. L., Peterson, J. A., Fligiel, S., Jolly, G., and Heppner, G. H., 1981, Epithelial characteristics of five subpopulations of a heterogeneous strain BALB/cfC3H mouse mammary tumor.Cancer Research,41, 1720–1730.
Kripke, M. L., Gruys, E., and Fidler, I. J., 1978, Metastatic heterogeneity of cells from an ultraviolet light-induced murine fibrosarcoma of recent origin.Cancer Research,38, 2962–2967.
Mantovani, A., Giavazzi, R., Alessandri, G., Spreafico, F., and Garattini, S., 1981, Characterization of tumor lines derived from spontaneous metastases of a transplanted murine sarcoma.European Journal of Cancer,17, 71–76.
Nanni, P., De GIovannni, C., Lollini, P.-L., Nicoletti, G., and Prodi, G., 1983, TS/A: a new metastasizing cell line from a BALB/c spontaneous mammary adenocarcinoma.Clinical & Experimental Metastasis,1, 373–380.
Stackpole, C. W., 1981, Distinct lung-colonizing and lung-metastasizing cell populations in B16 mouse melanoma.Nature,289, 798–800.
Suzuki, N., 1983, Spontaneous versus artificial lung metastasis: discrepant effect of whole-body irradiation in NFSA2ALM and NFSA1 SLM tumor systems.Journal of the National Cancer Institute,71, 835–839.
Tsuruo, T., Yamori, T., Naganuma, K., Tsukagoshi, S., and Sakurai, Y., 1983, Characterization of metastatic clones derived from a metastatic variant of mouse colon adenocarcinoma 26.Cancer Research,43, 5437–5442.
Van Lamsweerde, A.-L., Henry, N., and Vaes, G., 1983, Metastatic heterogeneity of cells from Lewis lung carcinoma.Cancer Research,43, 5314–5320.
Wexler, H., 1966, Accurate identification of experimental pulmonary metastases.Journal of the National Cancer Institute,36, 641–645.
Whitehead, R. H., Bertoncello, I., Webber, L. M., and Pedersen, J. S., 1983, A new human breast carcinoma cell line (PMC42) with stem cell characteristics. I. Morphological characterization.Journal of the National Cancer Institute,70, 649–661.
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Lollini, PL., De Giovanni, C., Eusebi, V. et al. High-metastatic clones selectedin vitro from a recent spontaneous BALB/c mammary adenocarcinoma cell line. Clin Exp Metast 2, 251–259 (1984). https://doi.org/10.1007/BF00132932
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DOI: https://doi.org/10.1007/BF00132932