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Manipulation of the genes for poly-β-hydroxybutyric acid synthesis in Alcaligenes eutrophus

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Summary

In order to establish the molecular breeding system in Alcaligenes eutrophus producing poly-β-hydroxybutyric acid (PHB), phbCAB genes from A. eutrophus were recombined into the E. coli-A. eutrophus shuttle vector and directly transferred into A. eutrophus by the electroporation. In A. eutrophus transformants, recombinant plasmids were stably maintained and enzyme activities for PHB biosyntheses were elevated 1.4–2.7 fold by the cloned genes.

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Authors and Affiliations

  1. Department of Genetic Engineering, College of Natural Sciences Kyungpook National University, 702-701, Taegu, Korea

    Hae-Chul Park, Jin-Seo Park, Yong-Hyun Lee & Tae-Lin Huh

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  1. Hae-Chul Park
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  2. Jin-Seo Park
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  3. Yong-Hyun Lee
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  4. Tae-Lin Huh
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Park, HC., Park, JS., Lee, YH. et al. Manipulation of the genes for poly-β-hydroxybutyric acid synthesis in Alcaligenes eutrophus . Biotechnol Lett 17, 729–734 (1995). https://doi.org/10.1007/BF00130359

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  • DOI: https://doi.org/10.1007/BF00130359

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