Summary
A cellulose hydrolysate from Aspen wood, containing mainly glucose, was fermented into ethanol by a thermotolerant strain MSN77 of Zymomonas mobilis. The effect of the hydrolysate concentration on fermentation parameters was investigated. Growth parameters (specific growth rate and biomass yield) were inhibited at high hydrolysate concentrations. Catabolic parameters (specific glucose uptake rate, specific ethanol productivity and ethanol yield) were not affected. These effects could be explained by the increase in medium osmolality. The results are similar to those described for molasses based media. Strain MSN77 could efficiently ferment glucose from Aspen wood up to a concentration of 60 g/l. At higher concentration, growth was inhibited.
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Abbreviations
- S:
-
glucose concentration (g/l)
- X:
-
biomass concentration (g/l)
- P:
-
′ethanol concentration (g/l)
- C:
-
conversion of glucose (%)
- t:
-
fermentation time (h)
- qS :
-
specific glucose uptake rate (g/g.h)
- qp :
-
specific ethanol productivity (g/g.h)
- YINX/S:
-
biomass yield (g/g)
- Yp/S :
-
ethanol yield (g/g)
- μ:
-
specific growth rate (h-1)
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Kademi, A., Baratti, J. Effect of substrate concentration on ethanol production by Zymomonas mobilis on cellulose hydrolysate. Biotechnol Lett 18, 1019–1024 (1996). https://doi.org/10.1007/BF00129724
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DOI: https://doi.org/10.1007/BF00129724