Summary
An analysis of the amplification fragments polymorphism of DNA coming from different accessions of germplasm belonging to species and cultivars of the genus Vitis, was carried out using 40 primer decamers of arbitrary sequence. The RAPD profiles showed a great intraspecific diversity. In many cases a single primer produced a unique pattern for each species. A phylogram tree based upon presence/absence data of the principal DNA bands divided the species according to their geographical origins. The intraspecific polymorphism of DNA fragments was not sufficient for an unambiguous identification of Vitis vinifera cultivars but the RAPD profiles turned out to be highly reproducible. The high capacity of this technique to generate DNA markers offers a new possibility for the study of the genetic relationships in the genus Vitis.
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Abbreviations
- PCR:
-
Polymerase chain reaction
- RAPD:
-
Random amplified polymorphic DNA
References
Alleweldt, G., P., Spiegel-Roy & B., Reisch, 1990. Grapes (Vitis). In: J.N., Moore & J.R., BallingtonJr. (Eds), Genetic Resources of Temperate Fruit and Nut Crops, pp. 291–327. Int. Soc. Hort. Sci., Wageningen.
Bourquin, J.C., P., Tournier, L., Otten & B., Walter, 1992. Identification of sixteen grapevine rootstocks by RFLP analysis of nuclear DNA extracted from the wood. Vitis 31: 157–162.
Büscher, N., E., Zyprian & R., Blaich, 1993. Identification of grapevine cultivars by DNA analysis: pitfalls of random amplified DNA techniques using 10 mer primers. Vitis 32: 187–188.
Collins, G.G. & R.H., Symons, 1993. Polymorphism in grapeviae DNA detected by the RAPD PCR technique. Plant Molecular Biology Reporter 11: 105–112.
Doyle, J.J. & J.L., Doyle, 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bull. 19: 11–15.
Eynard, I., 1969. Ampelografia e metodi ampelometrici. Quad. Università Pisa 9: 1–38.
Galet, P., 1956. Cépages et vignobles de France I. Les vignes américanes. Dehan (Ed.), Montpellier.
Gogorcena, Y., S., Arulsekar, A.M., Dandekar & D.E., Parfitt, 1993. Molecular markers for grape characterization. Vitis 32: 183–185.
Jean-Jaques, I., A., Defontaine & J.N., Hallet, 1993. Characterization of Vitis vinifera cultivars by Random Amplified Polymorphic DNA markers. Vitis 32: 189–190.
Koller, B., A., Lehmann, J.M., McDermott & C., Gessler, 1993. Identification of apple cultivars using RAPD markers. Theor. Appl. Genet. 85: 901–904.
Olmo, H.P., 1976. Grapes. In: N.W., Simmonds (Ed.), Evolution of Crop Plants, pp. 294–298. Longman, London.
Striem, M.J., P., Spegel-Roy, G., Ben-Hayyim, J., Beckmann & D., Gidoni, 1990. Genomic DNA fingerprinting of Vitis vinifera by the use of multi-loci probes. Vitis 29: 223–227.
Subden, R.E., A., Krizus, S.C., Lougheed & K., Carey, 1987. Isozyme characterization of Vitis species and some cultivars. Am. J. Enol. Vitic. 38: 176–181.
Thomas, M.R. & N.S., Scott, 1993. Microsatellite repeats in grapevine reveal DNA polymorphisms when analysed as sequence-tagged sites (STSs). Theor. Appl. Genet. 86: 985–990.
Weeden, N.F., B.I., Reisch & M.E., Martens, 1988. Genetic analysis of isozyme polymorphism in grape. J. Amer. Soc. Hort. Sci. 113: 765–769.
Welsh, J. & M., McClelland, 1990. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res. 18: 7213–7218.
Williams, J.G.K., A.R., Kubelik, K.J., Livak, J.A., Rafalski & S.V., Tingey, 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18: 6531–6535.
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Stella Grando, M., De Micheli, L. & Scienza, A. Characterization of Vitis germplasm using random amplified polymorphic DNA markers. Genet Resour Crop Evol 43, 187–192 (1996). https://doi.org/10.1007/BF00126763
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DOI: https://doi.org/10.1007/BF00126763