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Distamycin A/DAPI staining of heterochromatin in male meiosis of man

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Abstract

The sequential staining with distamycin A/DAPI provides an ideal method for studying the behaviour of heterochromatic regions in human male meiosis. The various meiotic and postmeiotic stages were found to have different staining qualities. Although all heterochromatic regions in human pachytene cells show specific DA/DAPI fluorescence, bright and clearly stained heterochromatic blocks can be distinguished from small DA/DAPI spots. Pachytene nuclei exhibit associations between heterochromatic regions of non-homologous bivalents. The heterochromatin of bivalent 9 generally presents as a cluster of small, discrete bodies. The heterochromatic regions of chromosomes 1, 9, 15, 16 and Y are preferentially stained at diakinesis and metaphase of the second meiotic division. The specific DA/DAPI staining disappears with the progressive volume reduction of middle and late spermatid nuclei. The heterochromatin of the chromatids fuses to form a large chromocenter during spermatid differentiation.

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References

  • Barr, H. J. & Ellison, J. R., 1972. Ectopic pairing of chromosome regions containing chemically similar DNA. Chromosoma 39: 53–61.

    Google Scholar 

  • Buckton, K. E., Spowart, G., Newton, M. S. & Evans, H. J., 1985. Forty four probands with an additional ‘marker’ chromosome. Hum. Genet. 69: 353–370.

    Google Scholar 

  • Buhariwalla, F. & Blecher, S. R., 1983. A rapid DAPI-distamycin method for identification of the mouse Y chromosome. Cytogenet. Cell Genet. 35: 78–79.

    Google Scholar 

  • Burgoyne, P. S., 1982. Genetic homology and crossing over in the X and Y chromosomes of mammals. Hum. Genet. 61: 85–90.

    Google Scholar 

  • Chandley, A. C., Edmond, P., Christie, S., Gowans, L., Fletcher, J., Frackiewicz, A. & Newton, M., 1975. Cytogenetics and infertility in man. I. Karyotype and seminal analysis. Ann. hum. Genet. 39: 231–254.

    Google Scholar 

  • Chen, A. T. L. & Falek, A., 1971. Cytological evidence for the association of the short arms of the X and Y chromosomes in the human male. Nature 232: 555–556.

    Google Scholar 

  • Driscoll, D. J., Palmer, C. G. & Melman, A., 1979. Nonhomologous associations of C-heterochromatin at human male meiotic prophase. Cytogenet. Cell Genet. 23: 23–32.

    Google Scholar 

  • Hsu, T. C., Cooper, J. E. K., Mace, M. L. & Brinkley, B. R., 1971. Arrangement of centromeres in mouse cells. Chromosoma 34: 73–87.

    Google Scholar 

  • Hungerford, D. A., 1972. Pachytene maps of normal human chromosomes and an example of their application in the study of cytogenetic abnormalities. In: Perspectives in cytogenetics. (Eds S. W.Wright, B. F.Crandell & L.Boyer), pp. 187–195, Charles C. Thomas, Springfield, Illinois, USA.

    Google Scholar 

  • Leibundgut, B., Müller, H., Haller, M. H. & DaRugna, D., 1979. Bedeutung der Meiose-Untersuchung bei männlicher Infertilität. Schweiz. med. Wschr. 109: 1600–1609.

    Google Scholar 

  • Luciani, J. M., Devictor, M., Morazzani, M. R. & Stahl, A., 1977. Pachytene mapping of the C 9 and acrocentric bivalents in the human oocyte. Hum. Genet. 36: 197–204.

    Google Scholar 

  • Mayr, B., Schnedl, W. & Schleger, W., 1979. Paarungsverhalten der meiotischen Chromosomen beim Rind. Z. Tierzücht. Züchtungsbiol. 95: 112–117.

    Google Scholar 

  • Mayr, B., Schweizer, D. & Schleger, W., 1983. Characterization of the canine karyotype by counterstain-enhanced chromosome banding. Can. J. Genet. Cytol. 25: 616–621.

    Google Scholar 

  • Meredith, R., 1969. A simple method for preparing meiotic chromosomes from mammalian testis. Chromosoma 26: 254–258.

    Google Scholar 

  • Page, B., 1973. Identification of chromosome 9 in human male meiosis. Cytogenet. Cell Genet. 12: 254–263.

    Google Scholar 

  • Palmer, C. G., Yu, P. L., Wang, L. Y., Merritt, A. D., Conneally, P. M., Magenis, R. E. & Rivas, M. L., 1977. Segregation and assortment of C-band variants of chromosome 1, 9, 16. J. supramol. Struct. Suppl. 1: 113.

  • Pathak, S., 1983. The behaviour of X chromosomes during mitosis and meiosis. In: Cytogenetics of the mammalian X chromosome, Part A: Basic mechanisms of X chromosome behaviour. (Ed. A. A.Sandberg), pp. 67–106, Alan R. Liss, New York.

    Google Scholar 

  • Pfeiffer, R. A., 1974. Cell cultures from blood and bone marrow. In: Methods in human cytogenetics (Eds H. G.Schwarzacher & U.Wolf), pp. 1–37, Springer, Berlin.

    Google Scholar 

  • Robinson, J. A., Buckton, K. E., Spowart, G., Newton, M., Jacobs, P. A., Evans, H. J. & Hill, R., 1976. The segregation of human chromosome polymorphisms. Ann. Hum. Genet. 40: 113–122.

    Google Scholar 

  • Schmid, M. & Haaf, T., 1984. Distamycin A/DAPI bands and the effects of 5-azacytidine on the chromosomes of the chimpanzee, Pan troglodytes. Cytogenet. Cell Genet. 38: 192–199.

    Google Scholar 

  • Schmid, M., Solleder, E. & Haaf, T., 1984. The chromosomes of Micromys minutus (Rodentia, Murinae). I. Banding analysis. Cytogenet. Cell Genet. 38: 221–226.

    Google Scholar 

  • Schmid, M., Haaf, T., Ott, G., Scheres, J. M. J. C. & Wensing, J. A. B., 1986. Heterochromatin in the chromosomes of the gorilla: analyses with distamycin A/DAPI, D287/170, chromomycin A3, quinacrine and 5-azacytidine. Cytogenet. Cell Genet. 41: 71–82.

    Google Scholar 

  • Schnedl, W., Abraham, R., Förster, M. & Schweizer, D., 1981. Differential fluorescent staining of porcine heterochromatin by chromomycin A3/distamycin A/DAPI and D287/170. Cytogenet. Cell Genet. 31: 249–253.

    Google Scholar 

  • Schwarzacher, H. G., 1974. Preparation of metaphase chromosomes. In: Methods in human cytogenetics. (Eds H. G.Schwarzacher & U.Wolf), pp. 71–81, Springer, Berlin.

    Google Scholar 

  • Schwarzacher, T., Mayr, B. & Schweizer, D., 1984. Heterochromatin and nucleolus-organizer-region behaviour at male pachytene of Sus scrofa domestica. Chromosoma 91: 12–19.

    Google Scholar 

  • Schweizer, D., Ambros, P. & Andrle, M., 1978. Modification of DAPI banding on human chromosomes by prestaining with a DNA binding oligopeptide antibiotic, distamycin A. Expl Cell Res. 111: 327–332.

    Google Scholar 

  • Schweizer, D., Ambros, P., Andrle, M., Rett, A. & Fiedler, W., 1979. Demonstration of specific heterochromatic segments in the orangutan (Pongo pygmaeus) by a distamycin A/DAPI double staining technique. Cytogenet. Cell Genet. 24: 7–14.

    Google Scholar 

  • Schweizer, D., 1981. Counterstain-enhanced chromosome banding. Hum. Genet. 57: 1–14.

    Google Scholar 

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Authors and Affiliations

  1. Institut für Humangenetik, Universität Würzburg, Koellikerstraße 2, D-8700, Würzburg, Federal Republic of Germany

    T. Haaf & M. Schmid

  2. Labor Humangenetik, Departement Forschung, Kantonsspital, CH-4031, Basel, Switzerland

    H. Müller

Authors
  1. T. Haaf
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  2. H. Müller
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  3. M. Schmid
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Haaf, T., Müller, H. & Schmid, M. Distamycin A/DAPI staining of heterochromatin in male meiosis of man. Genetica 70, 179–185 (1986). https://doi.org/10.1007/BF00122184

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  • DOI: https://doi.org/10.1007/BF00122184

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