Abstract
The number of plants in the gazetted rare species Stylidium coroniforme was increased through micropropagation. A method was first developed using the common species S. brunonianum. It was found that for both species, rapid propagation could be obtained by excising shoots from sterile seedlings and inducing shoot proliferation on Murashige and Skoog medium supplemented with 1 μM BAP. Rooting was achieved using 1 μM IBA and over 100 plants of each species were successfully established in soil. Leaf pieces could also be used to initiate cultures. In media with 20–25 μM BAP and 1–5 μM IBA, leaf pieces of S. brunonianum, S. piliferum, S. caricifolium and S. crassifolium produced adventitious buds, thus providing another method of micropropagation.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Carlquist S (1976) New species of Stylidium and notes on Stylidiaceae from South Western Australia. Aliso 8:447–463
Clements MA (1981) The germination of Australian orchid seed. Proc Orchid Symp (13th Int. Bot. Congress, Sydney) p 5–8
Coates DJ, James SH (1979) Chromosome variation in Stylidium crassocephalum (Angiospermae: Stylidiaceae) and the dynamic co-adaption of its lethal system. Chromosoma (Berl.) 72:357–376
Hopper S (1983) New wildflowers from the Wongan Hills Wildlife District. Swans 13:10–14
James SH (1979) Chromosome numbers and genetic systems in the trigger plants of Western Australia (Stylidium; Stylidiaceae). Aust J Bot 27:17–25
Kullmann WH (undated) Seed Germination Records of Western Australian Plants. Kings Park Research Notes No. 7
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Rye B, Hopper SD (1981) A Guide to the Gazetted Rare Flora of Western Australia. Department of Fisheries and Wildlife, Western Australia. Report No. 42
Ziv M, Meir G, Halevy AH (1983) Factors influencing the production of hardened glaucous carnation plantlets in vitro. Plant Cell Tissue Organ Culture 2:55–65
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
McComb, J.A. Micropropagation of the rare species Stylidium coroniforme and other Stylidium species. Plant Cell Tiss Organ Cult 4, 151–158 (1985). https://doi.org/10.1007/BF00042273
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00042273