Summary
Multiple shoots buds were obtained successfully from shoot tips of Acacia saligna by placing explants into solidified Murashighe & Skoog (1962) medium (MS medium) supplemented with 5.0 to 9.0 mg/L BAP. Sequential culture treatment was highly effective for shoot elongation using MS medium containing 0.3 mg/L BAP and 0.2 mg/L IAA. The shoots rooted best on MS medium supplemented with 2.0 mg/L IBA. Plantlet survival after transfer to soil was more than 90%. The shoot proliferation method described could be used for the mass clonal propagation of selected genotypes.
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Barakat, M.N., El-Lakany, M.H. Clonal propagation of Acacia saligna by shoot tip culture. Euphytica 59, 103–107 (1992). https://doi.org/10.1007/BF00041260
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DOI: https://doi.org/10.1007/BF00041260