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The presequence of the precursor to the nucleus-encoded 30 kDa protein of photosystem II in Euglena gracilis Z includes two hydrophobic domains

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Abstract

A cDNA clone for the extrinsic 30 kDa protein (OEC30) of photosystem II in Euglena gracilis Z was isolated and characterized. The open reading frame of the cDNA encoded a polypeptide of 338 amino acids, which consisted of a long presequence of 93 amino acids and a mature polypeptide of 245 amino acids. Two hydrophobic domains were identified in the presequence, in contrast to the presence of a single hydrophobic domain in the presequence of the corresponding proteins from higher plants. At the N- and C-terminal regions, respectively, of the presequence, a signal-peptide-like sequence and a thylakoid-transfer domain were identified. The presence of a long and unique presequence in the precursor to OEC30 is probably related to the complexity of the intracellular processes required for the synthesis and/or transport of the protein in Euglena.

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Abbreviations

ER:

endoplasmic reticulum

cDNA:

complementary DNA

SSU:

small subunit; Rubisco, ribulose 1,5-bisphosphate carboxylase/oxygenase

Rubico, ribulose 1,5:

bisphosphate carboxylase/oxygenase

LHC II:

light-harvesting chlorophyll protein of photosystem II

PS II:

photosystem II

OEC30:

the extrinsic 30 kDa protein of photosystem II in Euglena

PCR:

polymerase chain reaction

SDS:

sodium dodecyl sulfate

TE:

a solution containing 10 mM Tris-HCl and 1 mM EDTA pH 8.0

SSPE:

a solution containing 0.15 M NaCl, 10 mM NaH2PO4 and 1 mM EDTA pH 7.4

SDS-PAGE:

sodium dodecyl sulfate-polyacrylamide gel electrophoresis

PVDF:

poly(vinylidene difluoride)

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Authors and Affiliations

  1. Department of Biology, Faculty of Science, Okayama University, 700, Okayama, Japan

    Yasushi Shigemori, Junko Inagaki, Sumio Takahashi & Yasusi Yamamoto

  2. National Institute for Basic Biology, 444, Okazaki, Japan

    Hitoshi Mori & Mikio Nishimura

Authors
  1. Yasushi Shigemori
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  2. Junko Inagaki
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  3. Hitoshi Mori
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  4. Mikio Nishimura
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  5. Sumio Takahashi
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  6. Yasusi Yamamoto
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Shigemori, Y., Inagaki, J., Mori, H. et al. The presequence of the precursor to the nucleus-encoded 30 kDa protein of photosystem II in Euglena gracilis Z includes two hydrophobic domains. Plant Mol Biol 24, 209–215 (1994). https://doi.org/10.1007/BF00040587

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  • DOI: https://doi.org/10.1007/BF00040587

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