Summary
Factors favouring callus proliferation and subsequent regeneration of plants from pollen grains of rice anthers (Oryza sativa L., cvx. Bahia, Girona, Balilla × Sollana and Sequial) were determined. Cultivar differences in response were found, such as a high rate of haploid plant regeneration.
In addition, isolated pollen grain culture was used to induce tissue proliferation outside the anther walls. The frequency of callus formation from isolated pollen grains was very low. It was necessary to preculture the anthers before pollen grain separation, in order to accomplish a successful development later. Root differentiation was observed in some of the obtained callus.
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Cornejo-Martin, M.J., Primo-Millo, E. Anther and pollen grain culture of rice (Oryza sativa L.). Euphytica 30, 541–546 (1981). https://doi.org/10.1007/BF00038780
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DOI: https://doi.org/10.1007/BF00038780