Abstract
Explants from young joints of mature plants of tuna (Opuntia ficus-indica Mill.) were cultured on Murashige and Skoog (MS) medium containing 8.8 μM benzyladenine (BA) and 0.5 μM naphthaleneacetic acid (NAA). Shoots produced were utilized as secondary explants. Each shoot was cut longitudinally from apex to base into two explants, and some of these explants were cut transversely into proximal and distal explants. The size and number of shoots produced was affected by size and position of the explant within its source. The shoots were rooted in vitro or ex vitro and plants were successfully established in soil from both rooting methods.
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Abbreviations
- AC:
-
activated charcoal
- BA:
-
benzyladenine
- IBA:
-
indolebutyric acid
- MS:
-
Murashige & Skoog (1962) medium
- NAA:
-
naphthaleneacetic acid
References
Amin M & Jaiswal VS (1987) Rapid clonal propagation of guava through in vitro proliferation of nodal explants of mature trees. Plant Cell Tiss. Org. Cult. 9: 235–244
Escobar HA, Villalobos VM & Villegas AM (1986) Opuntia micro-propagation by axillary proliferation. Plant Cell Tiss. Org. Cult. 7: 269–277
Kahane R, Rancillac M & dela Serve Teyssendier B (1992) Long term multiplication of onion (Allium cepa L.) by cyclic shoot regeneration in vitro. Plant Cell Tiss. Org. Cult. 28: 281–288
Matsuo E, Matsuzawa M, Sakata Y & Arisumi K (1989) Asexual propagation of variegated Lilium longiflorum ‘Chataro’. Scientia Hort. 39: 349–354
Mauseth JD (1976) Cytokinins and gibberellic acid-induced effects on the structure and metabolism of shoot apical meristems in Opuntia polyacantha (Cactaceae). Amer. J. Bot. 63: 1295–1301
Mauseth JD & Halperin W (1975) Hormonal control of organogenesis in Opuntia polyacantha (Cactaceae). Amer. J. Bot. 62: 869–877
Mohamed-Yasseen Y, Barringer SA & Splittstoesser WE (1995) A review of the uses of Opuntia spp. in Central/North America. J. Arid Envir. (in press)
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Russell CE & Felker P (1987) The prickly pear (Opuntia spp., Cactaceae): A source of human and animal food in semiarid regions. Econ. Bot. 41: 433–445
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Mohamed-Yasseen, Y., Barringer, S.A., Splittstoesser, W.E. et al. Rapid propagation of tuna (Opuntia ficus-indica) and plant establishment in soil. Plant Cell Tiss Organ Cult 42, 117–119 (1995). https://doi.org/10.1007/BF00037690
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DOI: https://doi.org/10.1007/BF00037690