Abstract
Elite trees of Commiphora wightii (Arnott) Bhandari were selected from the wild on the basis of their content of guggul, an oleoresin. The selected tree was micropropagated through forced axillary branching on Murashige and Skoog's (MS) medium supplemented with benzyladenine (BA) and kinetin. Highest frequency of shoot formation was achieved on MS medium supplemented with 17.8 μM BA, 18.6 μM kinetin, 100 mg l-1 glutamine, 10 mg l-1 thiamine HCL and 0.3% activated charcoal. Seasonal changes affected the shoot proliferating potential of the initial explants in vitro. Transfer of shoots to a medium containing a lower concentration of BA (1.8 μM) and kinetin (1.9 μM) before rooting markedly stimulated shoot elongation. Shoots could be rooted by treating them with both indoleacetic acid and indolebutryic acid for 24 h in darkness and transferring them to a low-salt basal medium with activated charcoal. After rooting, transfer to a half-strength White's (modified) medium was necessary for further development of the plantlet. Regenerated plantlets were successfully established in soil.
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Abbreviations
- AC:
-
activated charcoal
- BA:
-
benzyladenine
- IAA:
-
indoleacetic acid
- IBA:
-
indolebutyric acid
- 2-iP:
-
isopentenyladenine
- MS:
-
Murashige and Skoog's medium
- NAA:
-
α-naphthaleneacetic acid
- PG:
-
phloroglucinol
References
Anonymous (1950) The Wealth of India: Raw materials, Vol 11 (p. 313) Council of Scientific and Industrial Research, New Delhi
Bajaj AG & Dev S (1982) Chemistry of Ayurvedic Crude Drugs V Guggul resin from Commiphora mukul-5: Some new steroidal components and stereochemistry of Guggulsterol-1 at C-20 and C-22. Tetrahedron 38: 2949–2954
Barve DM & Mehta AR (1989) Clonal propagation of Commiphora wightii by shoot tip cultures. In: Shah RR & Kukadia MU (Eds) Proc. Natl. Seminar on Agricultural Biotechnology, Navsari (pp 53–57) Gujarat Agricultural University, Navsari
Gupta PK, Nadgir AL, Mascarenhas AF & Jagannathan V (1980) Tissue culture of forest trees: clonal multiplication of Tectona grandis L. (Teak) by tissue culture. Plant Sci. Lett. 17: 259–268
Hu CY & Wang PJ (1983) Meristem, shoot tip and bud culture. In: Evans DA, Sharp WR, Ammirato PV & Yamada Y (Eds) Handbook of Plant Cell Culture, Vol 1 (pp 177–227) MacMillan Publ. Co., New York
Jaiswal VS & Amin MN (1987) In vitro propagation of guava from shoot cultures of mature trees. J. Plant Physiol. 130: 7–12
Malhotra CL, Agarwal YK, Mehta VL & Prasad S (1970) The effect of various fractions of gum guggul on experimentally produced hypercholesterolemia in chicks. Ind. J. Med. Res. 58: 394–395
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Nityanand S & Kapoor NK (1971) Hypercholesterolemic effect of Commiphora mukul resin (guggul). Ind. J. Exp. Biol. 9: 376–377
Patil VD, Nayak UR & Dev S (1972) Chemistry of Ayurvedic Crude Drugs I Guggulu (Resin from Commiphora mukul-I: Steroidal constituents, Tetrahedron 28: 2341–2352
Ravishankar R & Jagadish KS (1989) Micropropagation of Indian rosewood by tissue culture. Ann. Bot. 64: 43–46
Satyavati GV, Dwarkanath C & Tripathi SN (1969) Experimental studies on the hypocholesterolemic effect of Commiphora mukul Engl (Guggul). Ind. J. Med. Res. 57: 1950–1962
Sutter E & Barker P (1985) In vitro propagation of mature Liquidambar styraciflua. Plant Cell Tiss Org. Cult. 5: 13–21
White PR (1954) The Cultivation of Animal and Plant Cells, Thomas and Hudson, London
Wood HN & Braun AC (1961) Studies on the regulation of certain essential biosynthetic systems in normal and crowngall tumor cells. Proc. Natl. Acad. Sci. USA 47: 1907–1913
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Barve, D.M., Mehta, A.R. Clonal propagation of mature elite trees of Commiphora wightii . Plant Cell Tiss Organ Cult 35, 237–244 (1993). https://doi.org/10.1007/BF00037276
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DOI: https://doi.org/10.1007/BF00037276