Skip to main content
SpringerLink
Log in

A preparative method for the isolation of the 43 kDa, 47 kDa and the D1-D2-Cyt b 559 species directly from thylakoid membranes

  • Rapid Communication
  • Published:
Photosynthesis Research Aims and scope Submit manuscript

Abstract

Using the non-ionic detergent dodecyl-β-D-maltoside we have developed a preparative method for the isolation of the 43 kDa, 47 kDa and D1-D2-Cyt b 559 species directly from thylakoid membranes. In contrast to previous procedures the photosynthetic membrane was exposed only to one mild detergent and that resulted in more stable preparations. The isolated species were examined spectroscopically and it was found that even under these mild conditions the D1-D2-Cyt b 559 did not retain the primary quinone QA.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Abbreviations

PS II:

Photosystem II

CF:

Coupling Factor

LHC:

Light Harvesting Complex

MES:

4-morpholine-ethanesulfonic acid

BIS-TRIS:

2-[Bis (2-hydroxyethyl) amino]-2-hydroxymethyl-propane-1,3-diol

SMN:

0.4 M sucrose/50 mM MES (pH6)/10 mM NaCl

References

  • Akabori K, Tsukamoto H, Tsukihara J, Nagatsuka T, Motokawa O and Toyoshima Y (1988) Biochim Biophys Acta 932: 345–357

    Google Scholar 

  • Barber J (1987) Photosynthetic reaction centers: a common link. TIBS 12 (August 1987) 321–326

    Google Scholar 

  • Ghanotakis DF, Demetriou DM and Yocum CF (1987) Isolation and characterization of an oxygen-evolving Photosystem II reaction center core preparation and a 28 kDa Chl a binding protein. Biochim Biophys Acta 891: 15–21

    Google Scholar 

  • Ghanotakis DF, de Paula JC, Demetriou DM, Bowlby NR, Petersen J, Babcock GT and Yocum CF (1989) Isolation and characterization of the 47 kDa protein and the D1-D2-Cyt b 559 complex. Biocim Biophys Acta 974: 44–53

    Google Scholar 

  • Ikeuchi M and Inoue Y (1988) A new 4.8 kDa polypeptide intrinsic to the PS II reaction center, as revealed by modified SDS-PAGE with improved resolution of low-molecular weight proteins. Plant Cell Physiol 29: 1233–1239

    Google Scholar 

  • Ikeuchi K, Takio K and Inoue Y (1989) N-terminal sequencing of Photosystem II low-molecular-mass proteins. FEBS Lett 242: 263–269

    Google Scholar 

  • Komiya H, Yeates TO, Rees DC, Allen JP and Feher G (1988) Structure of the reaction center from Rhodobacter sphaeroides R-26 and 2.4.1: Symmetry relations and sequence comparisons between different species. Proc Natl Acad Sci USA 85: 9012–9016

    Google Scholar 

  • Michel H, Epp O and Deisenhofer J (1986) Pigment-protein interactions in the photosynthetic reaction center from Rhodopseudomonas Viridis. EMBO J 5: 2445–2451

    Google Scholar 

  • Nanbo O and Satoh K (1987) Isolation of a Photosystem II reaction center consisting of D-1 and D-2 polypeptides and Cytochrome b-559. Proc Natl Acad Sci USA 84: 109–112

    Google Scholar 

  • Rochaix J-D, Dron M, Rahire M and Malnoe P (1984) Sequence homology between the 32 kDa and the D2 chloroplast membrane polypeptides of Chlamydomonas reinhardii. Plant Mol Biol 3: 363–370

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Chemistry, University of Crete, Iraklion, Crete, Greece

    Constantina Fotinou & Demetrios F. Ghanotakis

Authors
  1. Constantina Fotinou
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Demetrios F. Ghanotakis
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Fotinou, C., Ghanotakis, D.F. A preparative method for the isolation of the 43 kDa, 47 kDa and the D1-D2-Cyt b 559 species directly from thylakoid membranes. Photosynth Res 25, 141–145 (1990). https://doi.org/10.1007/BF00035463

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00035463

Key words

Search

Navigation