Abstract
Large aliquot water samples (30 ml) were enriched with nutrients, then gelled in the cold with Polycell (wallpapering paste) and incubated to derive Saprolegniaceae colonies and hence deduced spore counts, from water collections of about 1 litre. The Saprolegnia pathogen of fish was a component of the total recoveries; it was recognised on the basis of its secondary zoospore cyst ornamentation. Windermere water entering The Ferry House fish hatchery gave spore assays of 2 to 28 1−1 for the pathogen, a significant component of total Saprolegnia, which was 6–73 spores per litre.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Beakes, G. W. & J. L. Gay, 1981. Effects of streptomycin on the growth and sporulation of Saprolegnia spp. J. gen. Microbiol. 19: 361–371.
Bucke, D., G. D. Cawley, J. F. Craig, A. D. Pickering & L. G. Willoughby, 1979. Further studies of an epizootic of perch Perca fluviatilis L., of uncertain aetiology. J. Fish Dis. 2: 297–311.
Cantino, E. C. & G. F. Turian, 1959. Physiology and development of lower fungi (Phytomycetes). Annu. Rev. Microbiol. 13: 97–124.
Hallett, I. C. & M. W. Dick, 1981. Seasonal and diurnal fluctuations of oomycete propagule numbers in the free water of a freshwater lake. J. Ecol. 69: 671–692.
Johansson, N., K. M. Svensson & G. Fridberg, 1982. Studies on the pathology of ulcerative dermal necrosis (UDN) in Swedish salmon, Salmo salar L., and sea trout, Salmo trutta L., populations. J. Fish Dis. 5: 293–308.
Martin, W. W., 1981. Couchia circumplexa, a water mold parasitic in midge eggs. Mycologia 73: 1143–1157.
Pickering, A. D., L. G. Willoughby & C. B. McGrory, 1979. Fine structure of secondary zoospore cyst cases of Saprolegnia isolates from infected fish. Trans. br. mycol. Soc. 72: 427–436.
Roberts, R. J., W. M. Shearer, A. L. S. Munro & K. G. R. Elson, 1970. Studies on ulcerative dermal necrosis of salmonids. 2. The sequential pathology of the lesions. J. Fish Biol. 2: 373–378.
Seymour, R. L., 1970. The genus Saprolegnia. Nova Hedwigia 19: 1–124.
Stuart, M. R. & H. T. Fuller, 1968. Mycological aspects of diseased Atlantic salmon. Nature 217: 90–92.
Willoughby, L. G., 1962. The occurrence and distribution of reproductive spores of Saprolegniales in fresh water. J. Ecol. 50: 733–759.
Willoughby, L. G., 1968. Atlantic salmon disease fungus. Nature 217: 872–873.
Willoughby, L. G., 1972. U.D.N. of Lake District trout and char: outward signs of infection and defence barriers examined further. Salm. Trout Mag. 195: 149–158.
Willoughby, L. G., 1978. Saprolegnias of salmonid fish in Windermere: a critical analysis. J. Fish Dis. 1: 51–67.
Willoughby, L. G., & V. G. Collins, 1966. A study of the distribution of fungal spores and bacteria in Blelham Tarn and its associated streams. Nova Hedwigia 12: 150–171.
Willoughby, L. G. & J. W. Copland, in press. Temperature-growth relationships of Saprolegnia pathogenic to fish. especially eels grown in warm water. Nova Hedwigia.
Willoughby, L. G. & A. D. Pickering, 1977. Viable Saprolegniaceae spores on the epidermis of the salmonid fish Salmo trutta and Salvelinus alpinus. Trans. br. mycol. Soc. 68: 91–95.
Willoughby, L. G., C. B. McGrory & A. D. Pickering, 1983. Zoospore germination of Saprolegnia pathogenic to fish. Trans. br. mycol. Soc. 80: 421–435.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Willoughby, L.G., Pickering, A.D. & Johnson, H.G. Polycell-gel assay of water for spores of Saprolegniaceae (fungi), especially those of the Saprolegnia pathogen of fish. Hydrobiologia 114, 237–248 (1984). https://doi.org/10.1007/BF00031875
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00031875