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Domain conservation in several volvocalean cell wall proteins

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Abstract

Based on our previous work demonstrating that (SerPro)x epitopes are common to extensin-like cell wall proteins in Chlamydomonas reinhardtii, we looked for similar proteins in the distantly related species C. eugametos. Using a polyclonal antiserum against a (SerPro)10 oligopeptide, we found distinct sets of stage-specific polypeptides immunoprecipitated from in vitro translations of C. eugametos RNA. Screening of a C. eugametos cDNA expression library with the antiserum led to the isolation of a cDNA (WP6) encoding a (SerPro)x-rich multidomain wall protein. Analysis of a similarly selected cDNA (VSP-3) from a C. reinhardtii cDNA expression library revealed that it also coded for a (SerPro)x-rich multidomain wall protein. The C-terminal rod domains of VSP-3 and WP6 are highly homologous, while the N-terminal domains are dissimilar; however, the N-terminal domain of VSP-3 is homologous to the globular domain of a cell wall protein from Volvox carteri. Exon shuffling might be responsible for this example of domain conservation over 350 million years of volvocalean cell wall protein evolution.

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Authors and Affiliations

  1. Department of Biology, Washington University, Box 1229, 63130, St. Louis, MO, USA

    Jeffrey P. Woessner & Ursula W. Goodenough

  2. Department of Molecular Cell Biology, University of Amsterdam, Kruislaan 318, 1098 SM, Amsterdam, Netherlands

    Arthur J. Molendijk, Piet van Egmond, Frans M. Klis & Michel A. Haring

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  1. Jeffrey P. Woessner
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  2. Arthur J. Molendijk
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  5. Ursula W. Goodenough
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Woessner, J.P., Molendijk, A.J., van Egmond, P. et al. Domain conservation in several volvocalean cell wall proteins. Plant Mol Biol 26, 947–960 (1994). https://doi.org/10.1007/BF00028861

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