Summary
We have sequenced cDNA clones representing each of the three distinct groups of storage proteins of the cotton seed. Characteristics of their mRNAs and derived proteins are given. Dot matrix analysis of the nucleotide and amino acid sequences shows that 2 of these groups of proteins have a great deal of vestigial homology at low stringency and should be considered subfamilies of a single storage protein gene family. The remaining group is quite distinct and should be considered a separate multigene family. It also can be divived into 2 subfamilies based on the presence or absence of glycosyl residues and other sequence differences.
These proteins are processed to smaller species during embryogenesis, and all of the mature storage proteins of cotton can be traced back to these 2 gene families.
In view of these relationships we propose that these 2 families be called the α and β globulins of cotton storage proteins, each comprised of an A and B subfamily.
Similar content being viewed by others
References
Dure LS, Chlan CA: Cottonseed storage proteins: products of three gene families. In: van Vloten-Doting L, Groot GSP, Hall TC (eds) Molecular Form and Function of the Plant Genome. Plenum Press, New York, 1985, pp 67–79.
Galau GA, Chlan CA, Dure LS: Developmental biochemistry of cottonseed embryogenesis and germination XVI. Analysis of the principal cotton storage protein gene families with cloned cDNA probes. Plant Mol Biol 2: 189–198, 1983.
Maxam AM, Gilbert W: Sequencing end-labelled DNA with base-specific chemical cleavages. In: Grossman L, Moldane K. (eds) Methods in Enzymology, Vol 65, Academic Press, New York, 1980, pp 499–560.
Garoff H, Ansorge W: Improvements of DNA sequencing gels. Anal Biochem 115: 450–457, 1981.
Kyte J, doolittle RF: A simple method for displaying the hydropathic character of a protein. J Mol Biol 157: 105–132, 1982.
Borroto K, Dure LS: Sequence homologies among the storage protein of angiosperms. Submitted.
Fagan JB, Pastan I, de Crombrugghe B: Sequence rearrangement and duplication of single stranded fibronectin cDNA probably occurring during cDNA synthesis by AMV reverse transcriptase and E. coli DNA polymerase I. Nucl Aci Res 8: 3055–3064, 1980.
Laughon A, Scott MP: Sequence of a Drosophila segmentation gene: protein structure homology with DNA-binding proteins. Nature 310: 25–31, 1984.
Von Heijne G: Patterns of amino acids near signal sequence cleavage sites. Eur J Biochem 133: 17–21, 1983.
Mizuno K, Matsuo H: A novel protease from yeast with specificity towards paired basic residues. Nature 309: 558–560, 1984.
Dure LS, Chlan CA, Galau GA: Cottonseed storage proteins as a tool for developmental biology. In: Ciferri O, Dure LS (eds) Structure and Function of Plant Genomes. Plenum Press, New York, 1983, pp 113–121.
Chilton WS, Tempé J, Matzke M, Chilton M-D: Succinamopine: a new crown gall opine. J Bacteriol 157: 357–362, 1984.
Ullrick A, Coussens L, Hayflick JS, Dull TJ, Gray A, Tam AW, Lee J, Yarden Y, Liberman TA, Schlessinger J, Downward J, Mayes ELV, Whittle N, Waterfield MD, Seeburg PH: Human epidermal growth factor receptor cDNA sequence and aberrant expression of the amplified gene in A431 epidermoid carcinoma cells. Nature 309: 418–425, 1984.
Ebina Y, Ellis L, Jarnagin K, Edesy M, Grof L, Clauser E, Ou J, Masiarz F, Kan YW, Goldfine ID, Roth RA, Rutter WJ: The human insulin receptor cDNA: the structural basis for hormone-activated transmembrane signalling. Cell 40: 747–758, 1985.
Hollenberg SM, Weinberger C, Ong ES, Cerelli G, Oro A, Lebo R, Thompson EB, Rosenfeld MG, Evans RM: Primary structure and expression of a functional human glucocorticoid receptor cDNA. Nature 318: 635–641, 1985.
Chlan CA, Borroto K, Kamalay J, Dure LS: Genome organization of the storage proteins of cottonseed. Submitted.
Moreira MA, Hermondson MA, Larkins BA, Nielsen NC: Partial characterization of the acidic and basic polypeptides of glycinin. J Biol Chem 254: 9921–9926, 1979.
Casey R, March JF, Sanger E: N-terminal amino acid sequence of Beta-subunits of legumin fromPisum sativum. Phytochem 20: 161–163, 1981.
Lycett GW, Croy RRD, Shirsat AH, Boulter D: The complete nucleotide sequence of a legumin gene from pea (Pisum sativum). Nucl Acid Res 12: 4493–4506, 1983.
Simon AE, Tenbarger KM, Scofield SR, Finkelstein RR, Crouch ML: Nucleotide sequence of a cDNA clone ofBrassica napus 12S storage protein shows homology with legumin fromPisum sativum. Plant Mol Biol 5: 191–201, 1985.
Walburg G, Larkins BA: Oat seed globulin. Subunit characterization and demonstration of its synthesis as a precursor. Plant Physiol 72: 161–165, 1983.
Borroto K, Dure LS: Disulfide linkages between the β globulin storage proteins of cottonseed. Submitted.
Dure LS, Chlan CA: Developmental biochemistry of cottonseed embryogenesis and germination XII purification and properties of the principal storage proteins. Plant Physiol 68: 180–186, 1981.
Dure LS, Galau GA: Ibid XIII Regulation of the biosynthesis of the principal storage proteins. Plant Physiol 68: 187–194, 1981.
Fryxell PA: The Natural History of the Cotton Tribe. Texas A & M University Press, College Station.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Chlan, C.A., Pyle, J.B., Legocki, A.B. et al. Developmental biochemistry of cottonseed embryogenesis and germination XVIII cDNA and amino acid sequences of members of the storage protein families. Plant Mol Biol 7, 475–489 (1986). https://doi.org/10.1007/BF00020331
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00020331