Abstract
The effect of adenine (ADE), isopentyl alcohol (IA) and a cytokinin-producing bacterium, Azotobacter chroococcum, on the morphological plant characteristics of Raphanus sativus (radish) was studied in sand under axenic-inoculated conditions and in soil under glasshouse and field conditions. The application of the combination of 0.2 mg kg−1 ADE, 13 mg kg−1 IA plus the inoculum enhanced the dry weight of root and shoot tissues, leaf area and chlorophyll a content, to a much greater degree than when in the presence of the cytokinin precursors (ADE or IA) or the bacterium alone. Enhanced plant growth observed under axenic conditions upon the addition of ADE and IA indicated that the plant has the ability to assimilate and utilize ADE and IA for growth and metabolism. While the addition of the inoculum without precursors was also stimulatory, greater enhancement of plant growth was observed following the application of ADE, IA and A. chroococcum together being attributed primarily to the increase in microbial production of cytokinins within the rhizosphere.
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References
Arnon D I 1949 Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiol. 24, 1–15.
Azcón R and Barea J M 1975 Synthesis of auxins, giberellins, and cytokinins by Azotobacter vinelandii and Azotobacter beijerinkii related to effects produced on tomato plants. Plant and Soil 43, 609–619.
Barea J M and Brown M E 1974 Effects on plant growth produced by Azotobacter paspali related to synthesis of plant growth regulating substances. J. Appl. Bacteriol. 37, 583–593.
Brown M E 1976 Role of Azotobacter paspali in association with Paspalum notatum. J. Appl. Bacteriol. 40, 341–348.
Duncan D B 1955 Multiple range and multiple F tests. Biometrics 11, 1–42.
Fletcher R A and Arnold V 1986 Stimulation of cytokinins and chlorophyll synthesis in cucumber cotyledons by triademefon. Physiol. Plant. 66, 197–201.
Gonzalez-Lopez J, Salmeron V, Martinez-Toledo M V, Ballesteros F and Ramos-Cormenzana A 1986 Production of auxins, gibberelins and cytokinins by Azotobacter vinelandii ATCC 12837 in chemically defined media and dialyzed soil media. Soil Biol. Biochem. 18, 119–120.
Fletcher R A and McCullagh D 1971 Cytokinin-induced chlorophyll formation in cucumber cotyledons. Planta 101, 88–90.
Jagnow G 1987 Inoculation of cereal crops and forage grasses with nitrogen-fixing rhizosphere bacteria: Possible causes of success and failure with regard to yield response — A review. Z. Pflanzenernaehr. Bodenkd. 150, 361–368.
Kadowaki M, Sasaki M, Fujiwara 1979 The content of thiamin in germ-free plants and biosynthesis of thiamin in vivo in germ-free leaves under gnotobiotic conditions. Soil Sci. Plant Nutr. 25, 621–626.
Maass H and Klämbt D 1981 On the biogenesis of cytokinins in roots of Phaseolus vulgaris. Planta 151, 353–358.
Mikulovich T P and Kukins I M 1985 Influence of cytokinin, fusicoccin, and potassium on accumulation of chlorophyll and carotenoids in isolated pumpkin cotyledons. Fiz. Rast. 32, 143–152.
Macura J 1965 Interrelations between microorganisms and plant in the rhizosphere. In Plant-Microbes Relationships. Eds. JMacura and VVancura. pp 26–33. House of the Czechoslovak Academy of Sciences. Prague, Czechoslovakia.
Miura G A and Miller C O 1969 6-(γ, γ-Dimethylallylaminopurine as a precursor of zeatin). Plant Physiol. 44, 372–376.
Mudliar A and Bharti S 1984 Mechanisms of benzyladenine-induced chlorophyll synthesis in excised cucumber cotyledons. Biochem. Physiol. Pflanzen. 179, 345–349.
Murai N 1980 Cytokinin biosynthesis and its relationship to the presence of plasmids in strains of Corynebacterium fascians. In Metabolism and Molecular Activities of Cytokinins. Eds. JGuern and CPéaud-Lenoël. pp 1–26. Springer-Verlag, Berlin.
Nieto K F and Frankenberger W T Jr 1987 HPLC analysis of cytokinins produced by soil bacteria. Proceedings of the 14th Annual Plant Growth Regulator Society of America meeting, Honolulu, Hawaii. 2–6 August 1987.
Nieto K F and Frankenberger W TJr 1988 HPLC analysis of cytokinins. J. Liquid Chromatogr. 11, 2907–2925.
Nieto K F and Frankenberger W TJr 1989a Biosynthesis of cytokinins produced by Azotobacter chroococcum. Soil Biol. Biochem. 21, 967–972.
Nieto K F and Frankenberger W TJr 1989b Biosynthesis of cytokinins in soil. Soil Sci. Soc. Am. J. 53, 735–740.
Nieto K F and Frankenberger W TJr 1990 Microbial production of cytokinins. In Soil Biochemistry. Vol. 6. Eds. J MBollag and GStotzky. pp 191–248. Marcel Dekker, New York.
Starnes W J and Hadley H H 1965 Chlorophyll content of various strains of soybeans, Glycine max (L.) Merrill. Crop Sci. 5, 9–11.
Steel R G D and Torrie J H 1980 Principles and Procedures of Statistics — A Biometrical Approach. 2nd Ed., pp 173–177. McGraw-Hill Book Co. NY.
Stuchbury T, Palni L M S, Horgan R and Wareing P F 1979 The biosynthesis of cytokinins in crown gall tissue of Vinca rosea. Planta 147, 97–102.
Tien T M, Gaskins M H, and Hubbell D H 1979 Plant growth substances produced by Azospirillum brasilense and their effect on the growth of pearl millet (Pennisetum americanum L.). Appl. Environ. Microbiol. 37, 1016–1024.
Wareing P F and Phillips I D J 1981 Growth and Differentiation in Plants. 3rd Ed., pp 64–66. Pergamon Press.
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Nieto, K.F., Frankenberger, W.T. Influence of adenine, isopentyl alcohol and Azotobacter chroococcum on the growth of Raphanus sativus . Plant Soil 127, 147–156 (1990). https://doi.org/10.1007/BF00014421
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DOI: https://doi.org/10.1007/BF00014421