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The extraction and electrophoretic fractionation of soil humic substances

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Summary

Humic substances were extracted with six different solvents (acetone, dioxane, ethanol, formic acid plus lithium bromide, sodium hydroxide plus stannous chloride and tris-EDTA boric-acid buffer) from soil under permanent maize, crop rotation, grass (Eragrostis curvula) and natural veld. The extracts were fractionated by column electrophoresis. The fractionation was followed by ultraviolet absorption. It is postulated that the quantity of lowmolecular weight humic substances rather than total humic-substance concentration should give an indication of soil fertility. From the results obtained the relative fertility status of the soil under different vegetational cover were determined. re]19740117

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Author notes

  1. J. N. Eloff

    Present address: Botany Department, University of the O F S, Bloemfontein/OFS., Republic of South Africa

Authors and Affiliations

  1. Agricultural Research Institute of the Highveld Region, Potchefstroom/TVL., Republic of South Africa

    J. N. Eloff & F. W. Pauli

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  1. J. N. Eloff
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  2. F. W. Pauli
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D-69 Heidelberg, Dammweg 13, IV, Federal Republic of Germany

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Eloff, J.N., Pauli, F.W. The extraction and electrophoretic fractionation of soil humic substances. Plant Soil 42, 413–422 (1975). https://doi.org/10.1007/BF00010016

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