Skip to main content
Log in

Electrophoretic studies on three enzymes from Sarcocystis species in sheep

  • Published:
Systematic Parasitology Aims and scope Submit manuscript

Summary

Thin-layer starch gel electrophoresis was used to distinguish isoenzymic differences between three species of Sarcocystis from sheep, Sarcocystis tenella, S. gigantea and S. medusiformis. Three enzymes, NADP dependent glutamate dehydrogenase, phosphoglucomutase and 6-phosphogluconate dehydrogenase showed distinctive enzyme banding patterns for the three species. ac]19800301

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Al-Taqi, M. & Evans, M.P. (1978) Characterization of Leishmania species from Kuwait by isoenzyme electrophoresis. Transactions of the Royal Society of Tropical Medicine and Hygiene, 72, 56–65.

    Google Scholar 

  • Atkinson, E.M. (1977) The use of electrophoresis in the identification of Sarcocystis. Vth International Congress of Protozology, New York, 1977, p. 421.

  • Carter, R. (1978) Studies on enzyme variation in murine malaria parasites P. berghei, P. yoelii, P. vinckei and P. chabaudi by starch gel electrophoresis. Parasitology, 76, 241–267.

    Google Scholar 

  • Carter, R. & McGregor, I.A. (1973) Enzyme variation in P. falciparum in the Gambia. Transactions of the Royal Society of Tropical Medicine and Hygiene, 67, 830–837.

    Google Scholar 

  • Carter, R. & Voller, A. (1973) Enzyme variants in primate malaria parasites. Transactions of the Royal Society of Tropical Medicine and Hygiene, 67, 14–15.

    Google Scholar 

  • Carter, R. & Voller, A. (1975) The distribution of enzyme variation in populations of P. falciparum in Africa. Transactions of the Royal Society of Tropical Medicine and Hygiene, 69, 371–376.

    Google Scholar 

  • Collins, G.H., Atkinson, E.M. & Charleston, W.A.G. (1979) Studies on Sarcocystis species. III The Macrocystic species in sheep. New Zealand Veterinary Journal, 27, 204–206.

    Google Scholar 

  • Collins, G.H., Charleston, W.A.G. & Moriarty, K.M. (1976) Sarcocystis species in sheep. New Zealand Veterinary Journal, 24, 123–124.

    Google Scholar 

  • Ford, G.E. (1974) Prey-predator transmission in the epizootiology of ovine sarcosporidiosis. Australian Veterinary Journal, 50, 38–39.

    Google Scholar 

  • Gardener, P.J., Chance, M.L. & Peters, W. (1974) Biochemical taxonomy of Leishmania. II Electrophoretic variation of MDH. Annals of Tropical Medicine and Parasitology, 68, 307–325.

    Google Scholar 

  • Gibson, W. (1977) Isoenzymes of T. brucei complex trypanosomes. Transactions of the Royal Society of Tropical Medicine and Hygiene, 71, 386.

    Google Scholar 

  • Godfrey, D.G. & Kilgour, V. (1976) Enzyme electrophoresis in characterizing the causative organism of Gambian trypanosomiasis. Transactions of the Royal Society of Tropical Medicine and Hygiene, 70, 219–224.

    Google Scholar 

  • Kilgour, V. & Godfrey, D.G. (1973) Species-characteristic isoenzymes of two aminotransferases in the trypanosomes. Nature, 244, 69–70.

    Google Scholar 

  • Kilgour, V., Godfrey, D.G. & Na'isa, B.K. (1975) Isoenzymes of two aminotransferases among Trypanosoma vivax in Nigerian cattle. Annals of Tropical Medicine and Parasitology, 69, 329–335.

    Google Scholar 

  • Levine, N.D. (1977) Nomenclature of Sarcocystis in the ox and sheep and of fecal coccidia of the dog and cat. Journal of Parasitology, 63, 36–51.

    Google Scholar 

  • Markus, M.B. Technique for the separation of Sarcocystis from cardic muscle. Journal of Parasitology, in press.

  • Peters, W., Chance, M.L., Mutinga, M.J., NGoka, J.M. & Schnur, L.F. (1977) The identification of human and animal isolates of Leishmania from Kenya. Annals of Tropical Medicine and Parasitology, 71, 501–502.

    Google Scholar 

  • Railliet, A. (1886a) Comment on: Moulé, L.T. (1886) “Psorospermies du tissu musculaire du mouton”. Bulletin et Mémoires de la Société Centrale de Médicine Vétérinaire, 40, 130.

    Google Scholar 

  • Railliet, A. (1886b) Psorospermies géantes dans l'oesophage et les muscles de mouton. Bulletin et Mémoires de la Société Centrale de Médicine Vétérinaire, 40, 130–134.

    Google Scholar 

  • Reeves, R.E. & Bischoff, J.M. (1968) Classification of Entamoeba species by means of electrophoretic properties of amoebal enzymes. Journal of Parasitology, 54, 594–600.

    Google Scholar 

  • Rollinson, D. (1975) Electrophoretic variation of enzymes in chicken coccidia. Transactions of the Royal Society of Tropical Medicine and Hygiene, 69, 436–437.

    Google Scholar 

  • Rollinson, D. (1976) Electrophoretic variation of enzymes in mammalian coccidia. Transactions of the Royal Society of Tropical Medicine and Hygiene, 70, 21–22.

    Google Scholar 

  • Rollinson, D. (1977) Biochemical and biological characters of Eimeria species. Ph.D. Thesis, University of London.

  • Sargeaunt, P.G., Williams, J.E. & Grene, J.D. (1978) The differentiation of invasive and non-invasive Entamoeba histolytica by isoenzyme electrophoresis. Transactions of the Royal Society of Tropical Medicine and Hygiene, 72, 519–521.

    Google Scholar 

  • Shaw, R.W. & Prasad, R. (1970) Starch-gel electrophoresis of enzymes—a compilation of recipes. Biochemical Genetics. 4, 297–320.

    Google Scholar 

  • Wraxall, B.G.D. & Culliford, B.J. (1968) A thin layer starch gel method for enzyme typing of blood stains. Journal of Forensic Science. 8, 81–82.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Atkinson, E.M., Collins, G.H. Electrophoretic studies on three enzymes from Sarcocystis species in sheep. Syst Parasitol 2, 213–216 (1981). https://doi.org/10.1007/BF00009533

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00009533

Keywords

Navigation