Abstract
A wide variety of toxins produced by cyanobacteria can cause severe health problems or even death in both people and animals. These cyanotoxins include neurotoxins, hepatotoxins, and dermatotoxins produced by the recurrent mass development of harmful algal blooms (HABs). Hepatotoxin microcystin and nodularin synthetase are the most commonly reported cyanotoxins. Aquatic food chains are known to be significantly impacted by microcystin bioaccumulation in aquatic life forms. Additionally, cyanotoxin biodegradation is very slow. Both microcystin and nodularin inhibit protein phosphate, lead to liver failure and hepatic hemorrhage. Consequently, it is rapidly developing into environmental and public concern. Thus, analysis of cyanotoxin gene clusters plays a vital role in identification of toxin-producing cyanobacteria. We describe a technique for locating gene clusters involved in the biosynthesis of cyanotoxins in the whole genome sequence of cyanobacterial species using the antiSMASH pipeline.
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Thajuddin, F., Rasheed, A.S., Thajuddin, N., Dhanasekaran, D. (2023). Identification of Microcystin, Nodularin Synthatase Gene Clusters in Toxic Cyanobacteria Using AntiSMASH Pipeline. In: Thajuddin, N., Sankara narayanan, A., Dhanasekaran, D. (eds) Protocols for Cyanobacteria Sampling and Detection of Cyanotoxin . Springer, Singapore. https://doi.org/10.1007/978-981-99-4514-6_53
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DOI: https://doi.org/10.1007/978-981-99-4514-6_53
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