Abstract
Apart from the singular exception of rhodopsin, GPCRs are naturally poorly abundant in the human body. As such, producing milligram amounts of purified, active, and stable GPCRs is not trivial and has long been considered challenging work to realize. With persistent technological and methodological efforts, a growing number of recombinant and active GPCRs has been successfully purified. In this chapter, we aim to discuss the solubilization and stabilization of GPCR using different detergents and styrene-maleic acid–lipid particles. We also highlight the commonly used purification techniques for GPCRs in literature. Finally, the heterologous expression systems for GPCRs, ranging from bacteria and yeasts to insects, mammalian cells, and Drosophila melanogaster, and the successful purification cases are reviewed.
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Abbreviations
- GPCRs:
-
G-protein coupled receptors
- CMCs:
-
Critical micelle concentrations
- HLB:
-
Hydrophile-lipophile balance
- DM:
-
n-Dodecyl-β-D-maltoside
- DDM:
-
n-Dodecyl-β-D-maltopyranoside
- LMNG:
-
Lauryl maltose neopentyl glycol
- CHS:
-
Cholesteryl hemisuccinate
- CHAPS:
-
3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate
- SMA:
-
Styrene-co-maleic acid
- SMALPs:
-
Styrene-co-maleic acid lipid particles
- DIBMA:
-
Diisobutylene maleic acid copolymer
- SMI:
-
Styrene-N-phenylmaleimide copolymer
- PMA:
-
Acrylic acid and maleic acid copolymer
- Twin-Strep:
-
10 × His
- Strep:
-
6 × His
- IEX:
-
Ion exchange chromatography
- GF:
-
Gel filtration
- HIC:
-
Hydrophobic interaction
- RPC:
-
Reversed phase chromatography
- GFP:
-
Green fluorescent protein
- MOI:
-
Multiplicity of infection
- PRCs:
-
Photoreceptor cells
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Zhao, X., Li, Q., Wang, J., Liang, Q., Quan, J. (2023). Purification of G Protein-Coupled Receptors. In: G Protein-Coupled Receptors . SpringerBriefs in Molecular Science. Springer, Singapore. https://doi.org/10.1007/978-981-99-0078-7_2
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