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Demonstration of Application of Fungal Xylanase in Fruit Juice and Paper Deinking and Validation of Its Mechanism Via In Silico Investigation

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Thermochemical and Catalytic Conversion Technologies for Future Biorefineries

Part of the book series: Clean Energy Production Technologies ((CEPT))

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Abstract

India is the leading provider of agricultural and forestry-based sustainable resources, that are typically considered waste, that is why developing a low-cost method for xylanase production holds great promise for converting discarded and underutilized lignocellulosic biomass (LCB) into usable products. In-house fungal cultures of Rhizomucor variabilis (Ac. No. KC602326.1) and Penicillium sp., (Ac. No. FJ430745.1) produced 18.3 IU/mL and 66.5 IU/mL of xylanase using rice straw as a carbon source. Furthermore, xylanase showed promising potential for use in the clarification of fruit juice as the enzyme was proved to be efficient in increasing reducing sugar, filterability, and clarity (%) from fruit juices. The paper industry has long been known as one of the major reasons for environmental pollution due to its high energy and chemical usage. The use of xylanases in the paper industry has been correlated to less pollution caused by the use of large amounts of chlorine and chlorinated compounds during pulp bleaching. Because of the depletion of fossil fuels, there is an urgent need to develop eco-friendly and long-term energy sources. As a result, the use of low-cost lignocellulosic materials is paramount for the cost-effective production of xylanase. In silico tools were also used to compare the results of wet lab experiments by docking xylanase with the ligands xylan, cellobiose, and lignin. In silico analysis of xylanase from the model fungal organisms had the highest affinity for xylan followed by cellobiose and lignin. Thus, it has future potential applications in the fruit juice clarification and paper and pulp industry.

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Abbreviations

BLAST:

Basic Local Alignment Search Tool

DNSA:

3, 5-dinitrosalicylic acid

FASTA:

Fast-All

GHG:

Greenhouse gases emissions

LCB:

Lignocellulosic biomass

LPCB:

Lactophenol cotton blue

NCBI:

National Center for Biotechnology Information

PDA:

Potato Dextrose Agar

PDB:

Protein Data Bank

SMR:

Swiss Model Repository

XOS:

Xylooligosaccharides

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Competing Interests

All the authors declare that they have no competing interests.

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Authors and Affiliations

  1. Department of Microbiology, Bioprocess & Bioenergy Lab, Central University of Rajasthan, Ajmer, Rajasthan, India

    Mamta Yadav, Komal Agrawal & Pradeep Verma

  2. Bioprocess and Bioenergy Laboratory, Department of Microbiology, Central University of Rajasthan, Ajmer, Rajasthan, India

    Bikash Kumar

  3. Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam, India

    Bikash Kumar

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  1. Mamta Yadav
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  2. Komal Agrawal
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Correspondence to Pradeep Verma .

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Editors and Affiliations

  1. Department of Microbiology, Bioprocess & Bioenergy Lab, Central University of Rajasthan, Ajmer, Rajasthan, India

    Pradeep Verma

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Yadav, M., Agrawal, K., Kumar, B., Verma, P. (2022). Demonstration of Application of Fungal Xylanase in Fruit Juice and Paper Deinking and Validation of Its Mechanism Via In Silico Investigation. In: Verma, P. (eds) Thermochemical and Catalytic Conversion Technologies for Future Biorefineries. Clean Energy Production Technologies. Springer, Singapore. https://doi.org/10.1007/978-981-19-4316-4_10

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