Skip to main content
SpringerLink
Log in

Stem Cell-Derived Exosomes: A Promising Therapeutic Role in Animal Models with Colorectal Cancer

  • Reference work entry
  • First Online:
Handbook of Animal Models and its Uses in Cancer Research

  • 735 Accesses

Abstract

Colorectal cancer (CRC), one of the most common afflicted cancers in both men and women worldwide, has caused a significant mortality rate. Distant metastases and recurrence are the two primary factors that contribute to patient death. Recent information has confirmed the cancer stem cells (CSCs) play a critical role in the development of cancer. CSCs are subpopulation of cancer cells with the ability to differentiate in several directions, proliferate indefinitely, and self-renew itself. CSCs play a critical role in the progress and recurrence of CRC. CSC-focused research has been conducted on a variety of malignancies, including CRC. This has led to the discovery and understanding of genes involved in the generation and maintenance of stem cells pluripotency as well as markers for CSCs, including those specifically investigated in CRC. The effectiveness of the current treatment against this malignancy, especially in instances of advanced CRC, is still lacking despite the adoption of new drugs and extensive therapies. Explicit understanding of mechanisms and factors that contribute to metastasis in CRC may be useful for identifying specific therapeutic targets to improve existing therapy. In recent decades, new treatment options for cancer have been emerged as a result of the increasing acceptance of the cancer stem cell theory. Exosomes are nanosized vesicles that are released by all cells, and they are now the focus of cancer therapy. Colonic mesenchymal stem cells (MSCs) experience morphological and functional changes as a consequence of CRC-derived exosomes, which aid in the growth of tumors and the progression of their malignant nature. Exosomes have been used as a delivery system for anticancer drugs in CRC. This review aims to summarize recent findings up to date several animal-based studies using exosomes derived from stem cells in CRC.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 599.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book
USD 599.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  • Akyurekli C et al (2015) A systematic review of preclinical studies on the therapeutic potential of mesenchymal stromal cell-derived microvesicles. Stem Cell Rev Rep 11(1):150–160

    Article  CAS  Google Scholar 

  • Al-Sowayan BS, Al-Shareeda AT, Alrfaei BM (2020) Cancer stem cell-exosomes, unexposed player in tumorigenicity. Front Pharmacol 11:384

    Article  CAS  Google Scholar 

  • Bahr MM et al (2020) Preservation techniques of stem cells extracellular vesicles: a gate for manufacturing of clinical grade therapeutic extracellular vesicles and long-term clinical trials. Int J Vet Sci Med 8(1):1–8

    Article  Google Scholar 

  • Banerjee A et al (2021) Role of tumor specific niche in colon cancer progression and emerging therapies by targeting tumor microenvironment. Adv Exp Med Biol 1341:177–192

    Article  Google Scholar 

  • Baroni S et al (2016) Exosome-mediated delivery of miR-9 induces cancer-associated fibroblast-like properties in human breast fibroblasts. Cell Death Dis 7(7):e2312

    Article  CAS  Google Scholar 

  • Bieback K, Klüter H (2007) Mesenchymal stromal cells from umbilical cord blood. Curr Stem Cell Res Ther 2(4):310–323

    Article  CAS  Google Scholar 

  • Chen Q-N et al (2017) Long non-coding RNAs in anti-cancer drug resistance. Oncotarget 8(1):1925–1936

    Article  Google Scholar 

  • Cheng X et al (2018) Circular RNA VMA21 protects against intervertebral disc degeneration through targeting miR-200c and X linked inhibitor-of-apoptosis protein. Ann Rheum Dis 77(5):770–779

    Article  CAS  Google Scholar 

  • Choi H et al (2021) Biodistribution of exosomes and engineering strategies for targeted delivery of therapeutic exosomes. Tissue Eng Regen Med 18(4):499–511

    Article  CAS  Google Scholar 

  • Cleophas MC et al (2017) ABCG2 polymorphisms in gout: insights into disease susceptibility and treatment approaches. Pharmgenomics Pers Med 10:129–142

    CAS  Google Scholar 

  • Colombo M et al (2013) Analysis of ESCRT functions in exosome biogenesis, composition and secretion highlights the heterogeneity of extracellular vesicles. J Cell Sci 126(Pt 24):5553–5565

    CAS  Google Scholar 

  • Dai J et al (2020) Exosomes: key players in cancer and potential therapeutic strategy. Signal Transduct Target Ther 5(1):145

    Article  CAS  Google Scholar 

  • Dalerba P et al (2007) Phenotypic characterization of human colorectal cancer stem cells. Proc Natl Acad Sci U S A 104(24):10158–10163

    Article  CAS  Google Scholar 

  • Dallas NA et al (2009) Chemoresistant colorectal cancer cells, the cancer stem cell phenotype, and increased sensitivity to insulin-like growth factor-I receptor inhibition. Cancer Res 69(5):1951–1957

    Article  CAS  Google Scholar 

  • Daßler-Plenker J, Küttner V, Egeblad M (2020) Communication in tiny packages: exosomes as means of tumor-stroma communication. Biochim Biophys Acta Rev Cancer 1873(2):188340

    Article  Google Scholar 

  • De Toro J et al (2015) Emerging roles of exosomes in normal and pathological conditions: new insights for diagnosis and therapeutic applications. Front Immunol 6:203

    Google Scholar 

  • Deng H et al (2016) Role of long non-coding RNA in tumor drug resistance. Tumour Biol 37(9):11623–11631

    Article  CAS  Google Scholar 

  • Endzeliņš E et al (2017) Detection of circulating miRNAs: comparative analysis of extracellular vesicle-incorporated miRNAs and cell-free miRNAs in whole plasma of prostate cancer patients. BMC Cancer 17(1):730

    Article  Google Scholar 

  • Gardiner C et al (2016) Techniques used for the isolation and characterization of extracellular vesicles: results of a worldwide survey. J Extracell Vesicles 5:32945

    Article  Google Scholar 

  • Gong M et al (2017) Mesenchymal stem cells release exosomes that transfer miRNAs to endothelial cells and promote angiogenesis. Oncotarget 8(28):45200

    Article  Google Scholar 

  • Gu H et al (2016) Exosomes derived from human mesenchymal stem cells promote gastric cancer cell growth and migration via the activation of the Akt pathway. Mol Med Rep 14(4):3452–3458

    Article  CAS  Google Scholar 

  • Hannafon BN, Ding W-Q (2015) Cancer stem cells and exosome signaling. Stem Cell Investig 2:11

    Google Scholar 

  • Hao ZC et al (2017) Stem cell-derived exosomes: a promising strategy for fracture healing. Cell Prolif 50(5):e12359

    Article  Google Scholar 

  • Hewson C, Morris KV (2016) Form and function of exosome-associated long non-coding RNAs in cancer. Curr Top Microbiol Immunol 394:41–56

    Google Scholar 

  • Huang Z, Feng Y (2017) Exosomes derived from hypoxic colorectal cancer cells promote angiogenesis through Wnt4-induced β-catenin signaling in endothelial cells. Oncol Res 25(5):651–661

    Article  Google Scholar 

  • Huang X et al (2015) Exosomal miR-1290 and miR-375 as prognostic markers in castration-resistant prostate cancer. Eur Urol 67(1):33–41

    Article  CAS  Google Scholar 

  • Huang Y et al (2020) Exosomes derived from bone marrow mesenchymal stem cells promote osteosarcoma development by activating oncogenic autophagy. J Bone Oncol 21:100280

    Article  Google Scholar 

  • Juan T, Fürthauer M (2018) Biogenesis and function of ESCRT-dependent extracellular vesicles. Semin Cell Dev Biol 74:66–77

    Article  CAS  Google Scholar 

  • Kalimuthu S et al (2016) In vivo therapeutic potential of mesenchymal stem cell-derived extracellular vesicles with optical imaging reporter in tumor mice model. Sci Rep 6(1):30418

    Article  CAS  Google Scholar 

  • Kang Y, Pantel K (2013) Tumor cell dissemination: emerging biological insights from animal models and cancer patients. Cancer Cell 23(5):573–581

    Article  CAS  Google Scholar 

  • Kehat I et al (2001) Human embryonic stem cells can differentiate into myocytes with structural and functional properties of cardiomyocytes. J Clin Invest 108(3):407–414

    Article  CAS  Google Scholar 

  • Konala VB et al (2016) The current landscape of the mesenchymal stromal cell secretome: a new paradigm for cell-free regeneration. Cytotherapy 18(1):13–24

    Article  CAS  Google Scholar 

  • Kuismanen SA et al (2000) Genetic and epigenetic modification of MLH1 accounts for a major share of microsatellite-unstable colorectal cancers. Am J Pathol 156(5):1773–1779

    Article  CAS  Google Scholar 

  • Lai RC et al (2010) Exosome secreted by MSC reduces myocardial ischemia/reperfusion injury. Stem Cell Res 4(3):214–222

    Article  CAS  Google Scholar 

  • Lapidot T et al (1994) A cell initiating human acute myeloid leukaemia after transplantation into SCID mice. Nature 367(6464):645–648

    Article  CAS  Google Scholar 

  • Li P et al (2017) Progress in exosome isolation techniques. Theranostics 7(3):789–804

    Article  CAS  Google Scholar 

  • Li J et al (2018) Circular RNA IARS (circ-IARS) secreted by pancreatic cancer cells and located within exosomes regulates endothelial monolayer permeability to promote tumor metastasis. J Exp Clin Cancer Res 37(1):177

    Article  Google Scholar 

  • Liu Z et al (2019) Exosomes from adipose-derived mesenchymal stem cells prevent cardiomyocyte apoptosis induced by oxidative stress. Cell Death Discov 5:79

    Article  Google Scholar 

  • Mashouri L et al (2019) Exosomes: composition, biogenesis, and mechanisms in cancer metastasis and drug resistance. Mol Cancer 18(1):75

    Article  Google Scholar 

  • Mathivanan S et al (2012) ExoCarta 2012: database of exosomal proteins, RNA and lipids. Nucleic Acids Res 40(Database issue):D1241–D1244

    Article  CAS  Google Scholar 

  • Miller IV, Grunewald TG (2015) Tumour-derived exosomes: tiny envelopes for big stories. Biol Cell 107(9):287–305

    Article  CAS  Google Scholar 

  • Mimeault M, Batra SK (2014) Molecular biomarkers of cancer stem/progenitor cells associated with progression, metastases, and treatment resistance of aggressive cancers. Cancer Epidemiol Biomark Prev 23(2):234–254

    Article  CAS  Google Scholar 

  • Moreno-Gonzalo O, Fernandez-Delgado I, Sanchez-Madrid F (2018) Post-translational add-ons mark the path in exosomal protein sorting. Cell Mol Life Sci 75(1):1–19

    Article  CAS  Google Scholar 

  • Morikawa K et al (1988) In vivo selection of highly metastatic cells from surgical specimens of different primary human colon carcinomas implanted into nude mice. Cancer Res 48(7):1943–1948

    CAS  Google Scholar 

  • Muralikumar M et al (2021) Current understanding of the mesenchymal stem cell-derived exosomes in cancer and aging. Biotechnol Rep (Amst) 31:e00658

    Article  CAS  Google Scholar 

  • Nakamura Y et al (2015) Mesenchymal-stem-cell-derived exosomes accelerate skeletal muscle regeneration. FEBS Lett 589(11):1257–1265

    Article  CAS  Google Scholar 

  • Navarro M et al (2017) Colorectal cancer population screening programs worldwide in 2016: an update. World J Gastroenterol 23(20):3632–3642

    Article  Google Scholar 

  • Orlic D et al (2001) Bone marrow cells regenerate infarcted myocardium. Nature 410(6829):701–705

    Article  CAS  Google Scholar 

  • Puik JR et al (2017) miRNA profiling for diagnosis, prognosis and stratification of cancer treatment in cholangiocarcinoma. Pharmacogenomics 18(14):1343–1358

    Article  CAS  Google Scholar 

  • Qiu G et al (2019) Functional proteins of mesenchymal stem cell-derived extracellular vesicles. Stem Cell Res Ther 10(1):359

    Article  Google Scholar 

  • Riau AK et al (2019) Sustained delivery system for stem cell-derived exosomes. Front Pharmacol 10:1368

    Article  CAS  Google Scholar 

  • Roccaro AM et al (2013) BM mesenchymal stromal cell-derived exosomes facilitate multiple myeloma progression. J Clin Invest 123(4):1542–1555

    Article  CAS  Google Scholar 

  • Sandiford OA et al (2021) Mesenchymal stem cell-secreted extracellular vesicles instruct stepwise dedifferentiation of breast cancer cells into dormancy at the bone marrow perivascular region. Cancer Res 81(6):1567–1582

    Article  CAS  Google Scholar 

  • Schlüter K et al (2006) Organ-specific metastatic tumor cell adhesion and extravasation of colon carcinoma cells with different metastatic potential. Am J Pathol 169(3):1064–1073

    Article  Google Scholar 

  • Setti M et al (2015) Extracellular vesicle-mediated transfer of CLIC1 protein is a novel mechanism for the regulation of glioblastoma growth. Oncotarget 6(31):31413–31427

    Article  Google Scholar 

  • Shen Y, Cao D (2012) Hepatocellular carcinoma stem cells: origins and roles in hepatocarcinogenesis and disease progression. Front Biosci (Elite Ed) 4:1157–1169

    Article  Google Scholar 

  • Siegel RL, Miller KD, Jemal A (2020) Cancer statistics, 2020. CA Cancer J Clin 70(1):7–30

    Article  Google Scholar 

  • Smid M et al (2019) The circular RNome of primary breast cancer. Genome Res 29(3):356–366

    Article  CAS  Google Scholar 

  • Sun Z-P et al (2017) MicroRNA expression profiling in exosomes derived from gastric cancer stem-like cells. Oncotarget 8(55):93839–93855

    Article  Google Scholar 

  • Taha EA, Ono K, Eguchi T (2019) Roles of Extracellular HSPs as Biomarkers in Immune Surveillance and Immune Evasion. Int J Mol Sci 20(18):4588

    Article  CAS  Google Scholar 

  • Takebe N et al (2015) Targeting Notch, Hedgehog, and Wnt pathways in cancer stem cells: clinical update. Nat Rev Clin Oncol 12(8):445–464

    Article  CAS  Google Scholar 

  • Tang Y et al (2016) Radiation-induced miR-208a increases the proliferation and radioresistance by targeting p21 in human lung cancer cells. J Exp Clin Cancer Res 35:7–7

    Article  Google Scholar 

  • Tang X et al (2019) Tumour-secreted Hsp90α on external surface of exosomes mediates tumour – stromal cell communication via autocrine and paracrine mechanisms. Sci Rep 9(1):15108

    Article  Google Scholar 

  • Théry C et al (1999) Molecular characterization of dendritic cell-derived exosomes. Selective accumulation of the heat shock protein hsc73. J Cell Biol 147(3):599–610

    Article  Google Scholar 

  • Trams EG et al (1981) Exfoliation of membrane ecto-enzymes in the form of micro-vesicles. Biochim Biophys Acta 645(1):63–70

    Article  CAS  Google Scholar 

  • Wei D et al (2021) RAB31 marks and controls an ESCRT-independent exosome pathway. Cell Res 31(2):157–177

    Article  CAS  Google Scholar 

  • Welton JL et al (2010) Proteomics analysis of bladder cancer exosomes. Mol Cell Proteomics 9(6):1324–1338

    Article  CAS  Google Scholar 

  • Wu J et al (2017) Role of stem cell-derived exosomes in cancer. Oncol Lett 13(5):2855–2866

    Article  CAS  Google Scholar 

  • Xie F et al (2019) Extracellular vesicles in cancer immune microenvironment and cancer immunotherapy. Adv Sci 6(24):1901779

    Article  CAS  Google Scholar 

  • Xu J, Liao K, Zhou W (2018) Exosomes regulate the transformation of cancer cells in cancer stem cell homeostasis. Stem Cells Int 2018:4837370

    Article  Google Scholar 

  • Xu Y et al (2019) microRNA-16-5p-containing exosomes derived from bone marrow-derived mesenchymal stem cells inhibit proliferation, migration, and invasion, while promoting apoptosis of colorectal cancer cells by downregulating ITGA2. J Cell Physiol 234(11):21380–21394

    Article  CAS  Google Scholar 

  • Zhang L, Yu D (2019) Exosomes in cancer development, metastasis, and immunity. Biochim Biophys Acta Rev Cancer 1871(2):455–468

    Article  CAS  Google Scholar 

  • Zhang YM et al (2002) Stem cell-derived cardiomyocytes demonstrate arrhythmic potential. Circulation 106(10):1294–1299

    Article  Google Scholar 

  • Zhang X et al (2015) Exosomes in cancer: small particle, big player. J Hematol Oncol 8(1):83

    Article  Google Scholar 

  • Zhou R et al (2018) The decade of exosomal long RNA species: an emerging cancer antagonist. Mol Cancer 17(1):75

    Article  Google Scholar 

  • Zhu L et al (2020) Isolation and characterization of exosomes for cancer research. J Hematol Oncol 13(1):152

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Cancer Science Laboratory, Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Chennai, India

    Dibyashree Chhetri, Kanagaraj Palaniyandi & Dhanavathy Gnanasampanthapandian

  2. Department of Surgical Gastroenterology, SRM Medical College Hospital and Research Center, SRM Institute of Science and Technology, Chennai, India

    Rathnaswami Arunachalam

  3. Interdisciplinary Institute of Indian System of Medicine, SRM Institute of Science and Technology, Chennai, India

    Ganesh Munuswamy-Ramanujam & Rajappan Chandra Satish Kumar

Authors
  1. Dibyashree Chhetri
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Rathnaswami Arunachalam
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ganesh Munuswamy-Ramanujam
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Rajappan Chandra Satish Kumar
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Kanagaraj Palaniyandi
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Dhanavathy Gnanasampanthapandian
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Kanagaraj Palaniyandi or Dhanavathy Gnanasampanthapandian .

Editor information

Editors and Affiliations

  1. Cancer Biology and Biotechnology Lab Faculty of Allied Health Sciences Chettinad Hospital & Research Institute (CHRI), Chettinad Academy of Research and Education (CARE), Kelambakkam, Chennai, India

    Surajit Pathak

  2. Cancer Biology and Biotechnology Lab Faculty of Allied Health Sciences Chettinad Hospital & Research Institute (CHRI), Chettinad Academy of Research and Education (CARE), Kelambakkam, Chennai, India

    Antara Banerjee

  3. AGENTEM (Adana Genetic Diseases Diagnosis and Treatment Center) & Medical Genetics Department of Medical Faculty, Cukurova University, Adana, Turkey

    Atil Bisgin

Rights and permissions

Reprints and permissions

Copyright information

© 2023 Springer Nature Singapore Pte Ltd.

About this entry

Check for updates. Verify currency and authenticity via CrossMark

Cite this entry

Chhetri, D., Arunachalam, R., Munuswamy-Ramanujam, G., Kumar, R.C.S., Palaniyandi, K., Gnanasampanthapandian, D. (2023). Stem Cell-Derived Exosomes: A Promising Therapeutic Role in Animal Models with Colorectal Cancer. In: Pathak, S., Banerjee, A., Bisgin, A. (eds) Handbook of Animal Models and its Uses in Cancer Research. Springer, Singapore. https://doi.org/10.1007/978-981-19-3824-5_38

Download citation

Publish with us

Policies and ethics

Search

Navigation