Abstract
The combination chemotherapy with other anticancer techniques is of great clinical importance to reduce side effects and enhance the effectiveness of cancer treatment. Co-delivery of chemotherapy drugs and small interfering RNA (siRNA) through advanced nanotechnology provides a reliable and effective strategy for combination therapy with synergistic effect. In this regard, this protocol focuses on the fabrication of polypeptide cationic micelles and delivery of chemical drug and siRNA for synergistic antitumor therapy. Here, the preparation and characterization techniques of polypeptide cationic micelles are described. A triblock copolymer poly (ethylene glycol)-b-poly (L-lysine)-b-poly (L-leucine) (PEG-PLL-PLLeu) was prepared through ring-opening polymerization reaction. These cationic copolymers could effectively encapsulate the hydrophobic drug and negatively charged siRNA in a single nanomicelle for co-delivery of drug/gene. The co-delivery system of chemotherapy drugs (docetaxel, DTX) and siRNA (siRNA-Bcl-2) obviously reduced the expression of antiapoptotic Bcl-2 gene and synergistically promoted antitumor activity of DTX. The present results demonstrate that the polypeptide cationic micelle is an effective chemotherapeutic drug/siRNA co-delivery system, thereby further improving the combined therapeutic efficacy for tumor in vivo.
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Pan, H., Liu, L., Cai, L. (2022). Polypeptide Cationic Micelles–Mediated Co-delivery of Docetaxel and siRNA for Synergistic Tumor Therapy. In: Tian, H., Chen, X. (eds) Gene Delivery. Biomaterial Engineering. Springer, Singapore. https://doi.org/10.1007/978-981-16-5419-0_18
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DOI: https://doi.org/10.1007/978-981-16-5419-0_18
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