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Pharmacological Modeling of Gastrointestinal Disorders in Zebrafish for Drug Discovery and Development

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Zebrafish Model for Biomedical Research

Abstract

Zebrafish as a pharmacological model is gaining popularity due to its similarity in both transcriptional and developmental physiology with humans. In this chapter, zebrafish as a relevant model for gastrointestinal tract (GIT) diseases including inflammatory bowel disease (IBD) and GIT cancers is outlined. Information on the relevance of zebrafish models in cancer metastasis, host-microbe interactions, and screening of new drugs is also included in this chapter. In IBD, zebrafish as a tool is used to understand both the pathogenesis and treatment outcomes such as immune suppression or disease regression through drug screening. TgBAC, a transgenic zebrafish line, has revealed two major factors which results into IBD including depletion of epigenetic repression as well as excessive production of tumor necrosis factor. The features of human IBD disease pathology are observed in chemically induced zebrafish models, which are well established and commonly used to test the new chemical entities (NCEs). In addition, zebrafish models are also used to understand IBD immunology including expression of chemokine responses which are difficult to investigate in murine models and to understand intestinal development and GIT transit. Factors such as high fertility, low maintenance, and physiological and developmental similarities in the GIT with humans make zebrafish an efficient model to explore GIT cancers. In addition, comparative genomics to humans, transparent body, and rapidly developing embryos are prominent factors for the successful use of zebrafish models in GIT cancers.

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Acknowledgments

The authors are grateful to the Dean, Faculty of Veterinary Science, P.V. Narsimha Rao Telangana Veterinary University (PVNRTVU), Hyderabad, Telangana.

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  1. Nanobiotechnology Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology (IIT), Roorkee, Uttarakhand, India

    Vishakha Singh & Prince Allawadhi

  2. Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science (CVSc), PVNRTVU, Hyderabad, Telangana, India

    Akshata Patangrao Renushe, Dinesh Neeradi, Anil Kumar Banothu & Amit Khurana

  3. The Business Research Company (TBRC), Hyderabad, Telangana, India

    Sravan Kumar Pasumarthi

  4. Department of Pharmaceutical Chemistry, University Institute of Pharmaceutical Sciences (UIPS), Panjab University, Chandigarh, India

    Isha Khurana

  5. Institute of Molecular Pathobiochemistry, Experimental Gene Therapy and Clinical Chemistry (IFMPEGKC), RWTH Aachen University Hospital, Aachen, Germany

    Ralf Weiskirchen & Amit Khurana

  6. Department of Aquatic Animal Health Management, College of Fishery Science, PVNRTVU, Wanaparthy, Telangana, India

    Anil Kumar Banothu

  7. Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science (CVSc), PVNRTVU, Warangal, Telangana, India

    Kala Kumar Bharani & Amit Khurana

  8. Centre for Biomedical Engineering (CBME), Indian Institute of Technology (IIT) Delhi, New Delhi, India

    Amit Khurana

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  1. Department of Pharmacology, Symbiosis Medical College for Women, Pune, Maharashtra, India

    Prasan R. Bhandari

  2. Veterinary Pharmacology and Toxicology, College of Veterinary Science (CVSc), P. V. Narsimha Rao Telangana Veterinary University (PVNRTVU), Warangal, Telangana, India

    Kala Kumar Bharani

  3. Centre for Biomedical Engineering (CBME), Indian Institute of Technology (IIT) Delhi, New Delhi, Delhi, India

    Amit Khurana

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Singh, V. et al. (2022). Pharmacological Modeling of Gastrointestinal Disorders in Zebrafish for Drug Discovery and Development. In: Bhandari, P.R., Bharani, K.K., Khurana, A. (eds) Zebrafish Model for Biomedical Research . Springer, Singapore. https://doi.org/10.1007/978-981-16-5217-2_19

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