Abstract
The advent of molecular markers has revolutionized aquaculture through several genetic improvement programs. Since there is a growing demand for fish by the growing human population, the reduction in the fish genetic diversity can lead to its extermination. Molecular markers have proved beneficial not only in breeding programmes but also in planning conservation strategies based on genetic diversities of various fish species. These markers have been classified in various ways depending on the properties they possess such as dominant and codominant markers, type I and type II markers and linked and direct markers. The most commonly used markers in aquaculture include allozymes, Restriction Fragment Length Polymorphism (RFLP), Random Amplified Polymorphic DNA (RAPD), Amplified Fragment Length Polymorphism (AFLP), microsatellites, Expressed Sequence Tag (EST) and Single Nucleotide Polymorphism (SNP). SNPs are the most widely used markers in fish population genetics study since they are found in abundance. Microsatellite markers have also been developed in several commercially important fish species for desirable traits like growth enhancement, disease resistance, etc. Microsatellites have high Polymorphism Information Content (PIC) values for which they are considered very reliable. These molecular markers have application while comparing the wild and hatchery fish population. Hence for a sustainable management of fish resources it is imperative to utilize molecular markers.
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Change history
01 January 2022
The original version of this book was inadvertently published with the incorrect affiliation (Organizational Division) as “Reproduction” for authors Dr. Janmejay Parhi and Dr. Ananya Khatei in the Front Matter and Chapters 2, 5 and 11. It has been updated as “Fish Genetics and Reproduction”.
References
Ahmed MM, Ali BA, EI-Zaeem, S. Y. (2004) Application of RAPD markers in fish: part I—some genera (Tilapia, Sarotherodon and Oreochromis) and species (Oreochromis aureus and Oreochromis niloticus) of Tilapia. Int J Biotechnol 6(1):86–93
Botella S, Pujalte MJ, Macián MC, Ferrús MA, Hernández J, Garay E (2002) Amplified fragment length polymorphism (AFLP) and biochemical typing of Photobacterium damselae subsp. damselae. J Appl Microbiol 93(4):681–688
Chiu TH, Su YC, Pai JY, Chang HC (2012) Molecular markers for detection and diagnosis of the giant grouper (Epinephelus lanceolatus). Food Control 24(1–2):29–37
Fuchs H, Gross R, Stein H, Rottmann O (1998) Application of molecular genetic markers for the differentiation of bream (Abramis brama L.) populations from the rivers Main and Danube. J Appl Ichthyol 14(1–2):49–55
Häunfling B, Brandl R (2000) Phylogenetics of European cyprinids: insights from allozymes. J Fish Biol 57(2):265–276
Huang CF, Lin YH, Chen JD (2005) The use of RAPD markers to assess catfish hybridization. Biodivers Conserv 14(12):3003–3014
Li SF, Tang SJ, Cai WQ (2010) RAPD-SCAR markers for genetically improved NEW GIFT Nile Tilapia (Oreochromis niloticus niloticus L.) and their application in strain identification. Zool Res 31(2):147–153
Liu Z, Li P, Kucuktas H, Nichols A, Tan G, Zheng X et al (1999) Development of amplified fragment length polymorphism (AFLP) markers suitable for genetic linkage mapping of catfish. Trans Am Fish Soc 128(2):317–327
Lynch M, Milligan BG (1994) Analysis of population genetic structure with RAPD markers. Mol Ecol 3(2):91–99
Maldini M, Marzano FN, Fortes GG, Papa R, Gandolfi G (2006) Fish and seafood traceability based on AFLP markers: elaboration of a species database. Aquaculture 261(2):487–494
Nei M (1972) Genetic distance between populations. Am Nat 106(949):283–292
Ng SH, Chang A, Brown GD, Koop BF, Davidson WS (2005) Type I microsatellite markers from Atlantic salmon (Salmo salar) expressed sequence tags. Mol Ecol Notes 5(4):762–766
Raza S, Shoaib MW, Mubeen H (2016) Genetic markers: importance, uses and applications. Int J Sci Res Publ 6(3):221
Rexroad CE, Rodriguez MF, Coulibaly I, Gharbi K, Danzmann RG, DeKoning J et al (2005) Comparative mapping of expressed sequence tags containing microsatellites in rainbow trout (Oncorhynchus mykiss). BMC Genomics 6(1):54
Shikano T, Taniguchi N (2002) Using microsatellite and RAPD markers to estimate the amount of heterosis in various strain combinations in the guppy (Poecilia reticulata) as a fish model. Aquaculture 204(3–4):271–281
Vos P, Hogers R, Bleeker M, Reijans M, Lee TVD, Hornes M et al (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23(21):4407–4414
Williams JL (2005) The use of marker-assisted selection in animal breeding and biotechnology. Revue Scientifique et Technique 24(1):379
Wolf C, Burgener M, Hübner P, Lüthy J (2000) PCR-RFLP analysis of mitochondrial DNA: differentiation of fish species. LWT Food Sci Technol 33(2):144–150
Yue GH, Ho MY, Orban L, Komen J (2004) Microsatellites within genes and ESTs of common carp and their applicability in silver crucian carp. Aquaculture 234(1–4):85–98
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Khatei, A., Tripathy, P.S., Parhi, J. (2021). Molecular Markers in Aquaculture. In: Pandey, P.K., Parhi, J. (eds) Advances in Fisheries Biotechnology. Springer, Singapore. https://doi.org/10.1007/978-981-16-3215-0_11
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DOI: https://doi.org/10.1007/978-981-16-3215-0_11
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