Abstract
Melatonin-loaded PLA nanoparticles are nowadays important in biological system for its biodegradable nature for targeted drug delivery. Hence, aim of the study is to note applicability and toxicity of Mel-PLA nanoparticles in osteoporosis. Different concentrations of melatonin and PLA were prepared by dissolving in dichloromethane (DCM). The final dried nanoparticles were used for structural analysis by SEM, TEM, and FTIR. Toxicity and immunological impact of nanoparticles were evaluated on rats: control and nanoparticle treated (n = 5/group) for 7 days. Afterward animals were sacrificed and blood, liver, and kidney were collected. A fraction of blood was processed for TLC, DLC, and %LC, and the remaining was centrifuged at 3000x g at 4 °C for 30 min. Separated plasma was used for measurements of IL-2, IL-6, TNF-α, IFN-γ, IL-1β, urea, creatinine, and BUN. Both plasma and tissue homogenates were used for AST, ALT, ACP, and ALP estimations. We noted significantly high (p < 0.05) levels of TLC, DLC, %LC and IL-2, and TNF-α upon treatment. The rest of the parameters were found to be significantly low (IL-6, IL-1β, AST, ALT; p > 0.05) or unaffected (IFN-γ, ACP, and ALP). From our preliminary study, we may conclude that we have synthesized Mel-PLA nanoparticles and their effects were nontoxic to animals.
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Abbreviations
- ACP:
-
Acid phosphatase
- AFM:
-
Atomic force microscopy
- ALP:
-
Alkaline phosphatase
- ALT:
-
Alanine aminotransferase
- AST:
-
Aspartate aminotransferase
- DCM:
-
Dichloromethane
- DSC:
-
Differential scanning calorimetry
- FTIR:
-
Fourier-transform infrared spectroscopy
- IFN-γ:
-
Interferon gamma
- IL-1β:
-
Interleukin-1 beta
- IL-2:
-
Interleukin-2
- IL-6:
-
Interleukin-6
- LFT:
-
Liver function test
- Mel:
-
Melatonin
- PLA:
-
Poly lactic acid
- RFT:
-
Renal function test
- SEM:
-
Scanning electron microscopy
- TEM:
-
Transmission electron microscopy
- TNF-α:
-
Tumor necrosis factor-alpha
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Acknowledgment
Financial support to SG as Council for Scientific and Industrial Research (CSIR, New Delhi, India) as Research Associate (RA) is gratefully acknowledged. Authors are also grateful to Mr. Rishikesh K. Tiwari (UGC-SRF), Ms. Shikha Singh (UGC-SRF), and Ms. Upma Singh (UGC-JRF) for critical evaluation of the paper who also have helped significantly in improvising the quality of the same.
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Ghosh, S., Rai, S.K., Haldar, C., Pandey, R.S. (2020). Synthesis, Characterization, and Evaluation of Toxicity of Melatonin-Loaded Poly (D,L-Lactic Acid) Nanoparticles (Mel-PLA-Nanoparticles) and Its Putative Use in Osteoporosis. In: Mishra, P., Mishra, R.R., Adetunji, C.O. (eds) Innovations in Food Technology. Springer, Singapore. https://doi.org/10.1007/978-981-15-6121-4_27
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