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Germplasm Characterization: Utilizing the Underexploited Resources

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Genetic Mapping and Marker Assisted Selection

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Abstract

This chapter describes the ways and means of utilizing the different types of plant germplasm using traditional and trendy phenotyping procedures and how to incorporate the selected germplasm accessions into the molecular breeding program. Detailed step-by-step procedure of rice germplasm characterization is given as a case study. Besides, this chapter also focuses on basic considerations of allele mining and other advanced approaches in allele mining such as insertional mutagenesis, TILLING, Eco-TILLING, and Self-EcoTILLING as well as troubleshooting measures to overcome the challenges faced in allele mining. Later part of this chapter deals with different software available for genetic diversity analysis and statistical methods to be followed while using molecular markers for selecting appropriate parents for mapping population development. Finally, the use of DNA barcoding approaches in germplasm characterization and in other areas of agriculture is also outlined in this chapter.

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Author information

Authors and Affiliations

  1. Plant Biotechnology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India

    N. Manikanda Boopathi

Authors
  1. N. Manikanda Boopathi
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Critical Thinking Questions

Critical Thinking Questions

  1. 1.

    Why is it important to identify genetically diverse parents for genetic mapping of quantitative traits?

  2. 2.

    Genetic diversity analysis using dominant and co-dominant markers should be cautiously interpreted. Justify.

  3. 3.

    How can the results of DNA barcoding be integrated in the genetic mapping process?

  4. 4.

    Offer your views on using allele mining and promoter mining to genetically improve the crop for novel traits

  5. 5.

    Which one of the following has more impact on plant breeding: natural populations or man-made populations?

  6. 6.

    How to integrate different trends in phenomics in plant breeding program?

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Boopathi, N.M. (2020). Germplasm Characterization: Utilizing the Underexploited Resources. In: Genetic Mapping and Marker Assisted Selection. Springer, Singapore. https://doi.org/10.1007/978-981-15-2949-8_2

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