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Microbial Production of Industrial Proteins and Enzymes Using Metabolic Engineering

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Engineering of Microbial Biosynthetic Pathways

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Abstract

Metabolic engineering is a field of science, which takes advantage of previously gathered information about a particular pathway in a living organism and utilizes this for the improvement of product that could be either metabolite, enzyme, or any protein. Advances in various field of science specifically r-DNA technology, bioinformatics, synthetic biology, molecular genetics as well as other protein engineering technologies had given wings to metabolic engineering. Metabolic engineering has the capacity to mold the flux of a completely enzymatic pathway to a very newly designed pathway. It allows the modulation and production of either previously working metabolite or the production of a new novel enzyme in a different microbial strain. In the present era, there is huge demand of microbial enzymes and proteins for various purposes such as medication, oil and gas industry, dairy industry, baking industry, etc. Microbial strains are utilized as micro factories for the production of microbial enzymes and proteins via metabolic engineering. Therefore, in this book chapter we are dealing with the various criteria that are utilized for the selection of the strains, various approaches that are routinely utilized for the higher expression of genes, as well as various metabolic engineering strategies.

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Author information

Authors and Affiliations

  1. Gujarat Biotechnology Research Center, Department of Science and Technology, Government of Gujarat, Mahanagar Seva Sadan Building (MS Building), Gandhinagar, Gujarat, India

    Pooja Doshi & Manju Shri

  2. Gujarat Biotechnology Research Center, Department of Science and Technology, Gandhinagar, Gujarat, India

    Poonam Bhargava, Chaitanya G. Joshi & Madhvi Joshi

Authors
  1. Pooja Doshi
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  2. Manju Shri
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  3. Poonam Bhargava
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  4. Chaitanya G. Joshi
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  5. Madhvi Joshi
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Corresponding author

Correspondence to Madhvi Joshi .

Editor information

Editors and Affiliations

  1. Department of Biosciences, School of Science, Indrashil University, Mehsana, Gujarat, India

    Vijai Singh

  2. Department of Food Process Engineering, Sam Higginbottom University of Agriculture, Technology and Sciences, Allahabad, Uttar Pradesh, India

    Ajay Kumar Singh

  3. Gujarat Biotechnology Research Center, Department of Science and Technology, Gandhinagar, Gujarat, India

    Poonam Bhargava

  4. Gujarat Biotechnology Research Center, Department of Science and Technology, Gandhinagar, Gujarat, India

    Madhvi Joshi

  5. Gujarat Biotechnology Research Center, Department of Science and Technology, Gandhinagar, Gujarat, India

    Chaitanya G. Joshi

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Doshi, P., Shri, M., Bhargava, P., Joshi, C.G., Joshi, M. (2020). Microbial Production of Industrial Proteins and Enzymes Using Metabolic Engineering. In: Singh, V., Singh, A., Bhargava, P., Joshi, M., Joshi, C. (eds) Engineering of Microbial Biosynthetic Pathways. Springer, Singapore. https://doi.org/10.1007/978-981-15-2604-6_12

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