Abstract
Paper is a versatile platform for several biological assays. One of the most popular assays performed on paper substrates is the nucleic acid amplification test (NAAT). Compared to other diagnostic techniques, NAATs are specific, sensitive and fast. While the polymerase chain reaction (PCR) is still the most widely-used technique for amplifying nucleic acids, isothermal DNA amplification techniques have emerged as viable alternatives to PCR on paper. Many innovative detection techniques have also been employed to detect the amplified DNA on paper. There are three main aspects of nucleic acid analysis on paper, namely (a) sample preparation to extract the nucleic acids from cells, (b) amplification of DNA, and (c) detection of the amplified DNA. In this chapter, we will review various amplification techniques in detail. We will also briefly cover the mechanisms to detect the amplified nucleic acids on paper. Finally, we will discuss the potential of these technologies to be translated to the point of care.
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Naik, P., Manna, R., Paul, D. (2019). Nucleic Acid Amplification on Paper Substrates. In: Bhattacharya, S., Kumar, S., Agarwal, A. (eds) Paper Microfluidics. Advanced Functional Materials and Sensors. Springer, Singapore. https://doi.org/10.1007/978-981-15-0489-1_8
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