Abstract
Carbon nanotubes (CNTs) have gained considerable attention due to their exceptional properties including high surface area, excellent electrical, mechanical, and thermal properties. Since outer walls of CNTs are chemically inert and hydrophobic in nature, it is of primary importance to make them compatible for their use. Among various methods, oxidation of CNTs surfaces by simple acids provides a mean to immobilize biomolecules and new surfaces. Protein–CNT conjugates are being developed for a wide range of biomedical devices and therapies including drug delivery, cancer therapy, and biosensing applications. The ability of CNTs to promote electron transfer in various redox reactions suggests great promise for oxidases-based amperometric biosensor and this has led to the development of electrode modified with CNTs in the application of electrocatalytic oxidation of glucose, uric acid, cytochromec, nitric oxide, 4-aminophenol etc. Here our study presents the application of functionalized CNT for electro-conductive filmmaking using simple fabrication method. The fabricated CNT film has been used to study the immobilization of enzyme and its application as glucose biosensor. We have studied the surface morphology of CNT film by transmission electron microscopy (TEM) and Fourier-transform infrared (FTIR) spectrometer while the biosensor/ bioelectronic properties of the CNT film-based electrode was studied by bio-electrochemical techniques like cyclic voltammetry. The results suggest that CNT–polymer-based film can uniformly disperse the functionalized CNTs by molding methods which were found to be suitable for preparing transducers and it can be used for the measurement of glucose in an aqueous system.
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References
Zimmet, P., Alerti, K.G., Magliano, D.J., Bennett, P.H.: Diabetes mellitus statistics on prevalence and mortality: facts and fallacies. Nat. Rev. Endocrinol. 12, 616–622 (2016)
Saydah, S., Imperatore, G., Cheng, Y., Geiss, L.S., Albright, A.: Disparities in diabetes deaths among children and adolescents-United States, 2000–2014. Morb. Mortal. Wkly Rep. 66(19), 502–505 (2017)
Li, J., Zhang, D., Li, Y., Wu, J., Zhang, B.: Joint similar and specific learning for diabetes mellitus and impaired glucose regulation detection. Inf. Sci. 384, 191–204 (2017)
Galant, A.L., Kaufman, R.C., Wilson, J.D.: Glucose: detection and analysis. Food Chem. 188, 149–160 (2015)
Daines, T.L., Morse, K.W.: A spectrophotometric method for determination of glucose in blood serum. Logan 53, 126–127 (1976)
Dohnal, L., Kalousova, M., Zima, T.: Comparison of three methods for determination of glucose. Prague. Med. Rep. 111, 42–54 (2010)
Lin, T., Gal, A., Mayzel, Y., Horman, K., Bahartan, K.: Non-invasive glucose monitoring: a review of challenges and recent advances. Curr. Trends. Biomed. Eng. Biosci. 6(5), 001–008 (2017)
Goran, J.M., Mantilla, S.M., Stevenson, K.J.: Influence of surface adsorption on the interfacial electron transfer of flavin adenine dinucleotide and glucose oxidase at carbon nanotube and nitrogen-doped carbon nanotube electrodes. Anal. Chem. 85, 1571–1581 (2012)
Deng, C., Chen, J., Nie, Z., Si, S.: A sensitive and stable biosensor based on the direct electrochemistry of glucose oxidase assembled layer-by-layer at the multiwall carbon nanotube-modified electrode. Biosens. Bioelectron. 26, 213–219 (2010)
Tsai, T.W., Heckert, G., Neves, L.F., Tan, Y., Kao, D.Y., Harrison, R.G., Resasco, D.E., Schmidtke, D.W.: Adsorption of glucose oxidase onto single-walled carbon nanotubes and its application in layer-by-layer biosensors. Anal. Chem. 81, 7917–7925 (2009)
Xian, Y., Hu, Y., Liu, F., Xian, Y., Wang, H., Jin, L.: Glucose biosensor based on Au nanoparticles-conductive polyaniline nanocomposite. Biosens. Bioelectron. 21, 1996–2000 (2006)
Han, X., Zhu, Y., Yang, X., Li, C.: Amperometric glucose biosensor based on platinum nanoparticle encapsulated with a clay. Microchim. Acta 171, 233–239 (2010)
German, N., Ramanavicius, A., Ramanaviciene, A.: Amperometric glucose biosensor based on electrochemically deposited gold nanoparticles covered by polypyrrole. Electroanalysis 29, 1267–1277 (2017)
Guo, S., Dong, S.: Graphene nanosheet: synthesis, molecular engineering, thin film, hybrids, ad energy and analytical applications. Chem. Soc. Rev. 40, 2644–2672 (2011)
Gallay, P., Tosi, E., Madrid, R., Tirado, M., Comedi, D.: Gluocse biosensor based on functionalized ZnO nanowire/graphite films dispersed on a Pt electrode. Nanotechnology 27, 425501–425508 (2016)
Hui, Z.H., Hong, C., Hua, C.J., Fei, K.Y.: Polyaniline-graphite composite film glucose oxidase electrode. J. Cent. South Univ. Technol. 13, 653–657 (2006)
Zhang, H., Meng, Z., Wang, Q., Zheng, J.: A novel glucose biosensor based on direct electrochemistry of glucose oxidase incorporated in biomediated gold nanoparticles-carbon nanotubes composite film. Sens. Actuators. 158, 23–27 (2011)
Mani, V., Devadas, B., Chen, S.: Direct electrochemistry of glucose oxidase at electrochemically reduced grapheme oxide-multiwalled carbon nanotubes hybrid material modified electrode for glucose biosensor. Biosens. Bioelectron. 41, 309–315 (2013)
Gao, W., Tjiu, W.W., Wei, J., Liu, T.: Highly sensitive non enzymatic glucose and H2O2 sensor based on Ni(OH)2/electroreduced graphene oxide-Multiwalled carbon nanotube film modified glass carbon electrode. Talanta 120, 484–490 (2014)
Liu, J., Sun, S., Shang, H., Lai, J., Zhang, L.: Electrochemical biosensor based on bioenzyme and carbon nanotubes incorporated into an Os-complex thin film for continuous glucose detection in human saliva. Electroanalysis 28, 1–7 (2016)
Wu, Y., Hu, S.: Biosensors based on direct electron transfer in redox proteins. Microchim. Acta 159, 1–17 (2007)
Gokoglan, T.C., Soylemez, S., Kesik, M., Dogru, I.B., Turel, O., Yuksel, R., Unalan, H.E.: Toppare, L: a novel approach for the fabrication of a flexible glucose biosensor: The combination of vertically aligned CNTs and a conjugated polymer. Food Chem. 220, 299–305 (2017)
Baghayeri, M., Veisi, H., Ghanei-Motlagh, M.: Amperometric glucose biosensor based on immobilization of glucose oxidase on a magnetic glassy carbon electrode modified with a novel magnetic nanocomposite. Sens. Actuators B, 249, 321–330 (2017)
Jacobs, C.B., Peairs, M.J., Venton, B.J.: Review: carbon nanotube based electrochemical sensors for biomolecules. Anal. Chim. Acta 662, 105–127 (2010)
Shan, C., Yang, H., Han, D., Zhang, Q., Ivaska, A., Niu, L.: Graphene/AuNPs/chitosan nanocomposites film for glucose biosensing. Biosens. Bioelectron. 25, 1070–1074 (2010)
Saglam, O., Dilgin, Y.: Fabrication of photoelectrochemical glucose biosensor in flow injection analysis system using ZnS/CdS-Carbon nanotube nanocomposite electrode. Electroanalysis 29, 1–10 (2017)
Shrestha, B.K., Ahmad, R., Mousa, H.M., Kim, I., Kim, J., Neupane, M.P., Park, C.H., Kim, C.S.: High-performance glucose biosensosr based on chitosan-glucose oxidase immobilized ploypyrrole/Nafion/functionalized multi-walled carbon nanotubes bio-nanohybrid film. J. Colloid Interface Sci. 482, 39–47 (2016)
Saei, A.A., Najafi-Marandi, P., Abhari, A., Guardia, M., Dolatabadi, J.E.N.: Electrochemical biosensors for glucose based on metal nanoparticles. Trends. Anal. Chem. 00, 1–12 (2012). https://doi.org/10.1016/j.trac.2012.09.011
Gao, G., Luo, J., Ge, Z., Chen, S., Chen, S., Yang, H.: Self-assembling of electrochemical glucose biosensor with bacteriostatic materials via layer-by-layer method. J. Electrochem. Soc. 164, 189–192 (2017)
Wooten, M., Karra, S., Zhang, M., Gorski, W.: On the direct electron transfer, sensing and enzyme activity in the glucose oxidase/carbon nanotubes system. Anal. Chem. 86(1), 752–757 (2014). https://doi.org/10.1021/ac403250w
Turkmen, E., Bas, S.Z., Gulce, H., Yildiz, S.: Glucose biosensor based on immobilization of glucose oxidase in electropolymerized poly(o-phenylenediamine) film on platinum nanoparticles-polyvinylferrocenium modified electrode. Electrochimica Acta. 123, 93–102 (2014). https://doi.org/10.1016/j.electacta.2013.12.189
Prasad, R., Bhat, B.R.: Multi-wall carbon nanotube-NiO nanoparticle composite as enzyme-free electrochemical glucose sensor. Sens. Actuators B Chem. 220, 81–90 (2015). https://doi.org/10.1016/j.snb.2015.05.065
Liu, Y., Wang, M., Zhao, F., Xu, Z., Dong, S.: The direct electron transfer of glucose oxidase and glucose biosensor based on carbon nanotubes/chitosan matrix. Biosens. Bioelectron. 21, 984–988 (2005)
Luo, X., Xu, J., Wang, J., Chen, H.: Electrochemically deposited nanocomposite of chitosan and carbon nanotubes for biosensor application. Chem. Commun. 16, 2169–2171 (2005)
Vashist, S.K., Zheng, D., Al-Rubeaan, K., Luong, J.H.T., Sheu, F.: Advances in carbon nanotube based electrochemical sensors for bioanalytical applications. Biotechnol. Adv. 29, 169–188 (2011)
Wang, Y., Liu, L., Li, M., Xu, S., Gao, F.: Multifunctional carbon nanotubes for direct electrochemistry of glucose oxidase bioassay. Biosens. Bioelectron. 30, 107–111 (2011)
Lin, Y., Lu, F., Tu, Y., Ren, Z.: Glucose biosensors based on carbon nanotubes nanoelectrodes ensembles. Nano Lett. 4(2), 191–195 (2004)
Pan, D., Chen, J., Yao, S., Tao, W., Nie, N.: An amperometric glucose biosensor based on glucose oxidase immobilized in electropolymerized poly(o-aminophenol) and carbon nanotubes composite film on a gold electrode. Anal. Sci. 21, 367–371 (2005)
Saifuddin, N., Raziah, A.Z., Junizah, A.R.: Carbon nanotubes: a review on structure and their interaction with proteins. J. Chem. 013, 1–18 (2013)
Mobbott, G.A.: An introduction of cyclic voltammetry. J. Chem. Educ. 60, 697–702 (1983)
Acknowledgements
This work was supported by the Department of Biotechnology, New Delhi, India under a Bio-CARe women scientist scheme award to RS, [Grant No. BT/Bio-CARe/05/640/2011-12].
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Gopal, N., Shukla, P., Sahney, R. (2018). Development of CNT–Polymer Film-Based Electrode for the Detection of Glucose. In: Gupta, B., Ghosh, A., Suzuki, A., Rattan, S. (eds) Advances in Polymer Sciences and Technology. Materials Horizons: From Nature to Nanomaterials. Springer, Singapore. https://doi.org/10.1007/978-981-13-2568-7_15
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