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Conclusion and Future Outlooks

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Engineering a Robust DNA Circuit for the Direct Detection of Biomolecular Interactions

Part of the book series: Springer Theses ((Springer Theses))

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Abstract

This doctoral work culminated in a circuit design capable of detecting bi-molecular proximity events, which we named “split proximity circuit (SPC)”. The circuit assembles dynamically on the target recognition sites in a self-contained manner to execute localized strand displacement. A recurring theme in this thesis was to minimize circuit leakages and a major contribution of this thesis was the development of several key counter-leakage strategies which were demonstrated in two concepts of association toehold and hybridization chain reaction. These strategies should be useful for designing DNA circuits in general and particularly as a starting point for researchers outside the DNA circuitry field. In terms of assay development, the greatest advantages of the SPC are its operation simplicity and plug-and-play versatility when compared to existing detection technologies. Looking forward, the functionality of SPC can be expanded by modifying individual module, e.g. detection of live cells, using secondary antibody as the recognition moiety and incorporating a functional downstream output. Other scientific directions to be explored include the self-contained logic gate design on cell surfaces and enzymatic production of “defect-free” oligonucleotide strands.

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Authors and Affiliations

  1. Department of Chemical and Biomolecular Engineering, National University of Singapore, Singapore, 117585, Singapore

    Dr. Ang Yan Shan

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  1. Dr. Ang Yan Shan
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Yan Shan, A. (2018). Conclusion and Future Outlooks. In: Engineering a Robust DNA Circuit for the Direct Detection of Biomolecular Interactions. Springer Theses. Springer, Singapore. https://doi.org/10.1007/978-981-13-2188-7_9

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