Abstract
A number of protein-protein complexes are associated with disease progression and are considered as targets for drug and vaccine development. Therefore, it is important to understand protein-protein interfaces in these disease-linked complexes. We illustrate and discuss this phenomenon using a hexameric (six subunits) cholera toxin (CT) and a HIV-1/ENV GP160 (GP120/GP40) trimer (three subunits) spike protein complex. Analysis of subunit-subunit interfaces in these complexes play an important role in the design, development, and production of a stable yet a viable vaccine.
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References
Shamini G, Ravichandran M, Sinnott JT, Somboonwit C, Sidhu HS, Shapshak P, Kangueane P. Structural inferences for cholera toxin mutations in vibrio cholerae. Bioinformation. 2011;6(1):1–9.
Sowmya G, Shamini G, Anita S, Sakharkar M, Mathura V, Rodriguez H, Levine AJ, Singer E, Commins D, Somboonwit C, Sinnott JT, Sidhu HS, Rajaseger G, Pushparaj PN, Kangueane P, Shapshak P. HIV-1 envelope accessible surface and polarity: clade, blood, and brain. Bioinformation. 2011;6(2):48–56.
Dassault Systemes BIOVIA, BIOVIA Discovery Studio Visualizer, v16.1.0.15350, San Diego: Dassault Systemes, 2015 [cited: 2017 Mar 20]. Available from: http://accelrys.com/products/collaborative-science/biovia-discovery-studio/visualization-download.php
Tsodikov OV, Record MT Jr, Sergeev YV. Novel computer program for fast exact calculation of accessible and molecular surface areas and average surface curvature. J Comp Chem. 2002;23:600–9.
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Exercises
Exercises
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1.
Illustrate the structure of a functional cholera toxin.
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2.
How many subunits constitute a cholera toxin?
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3.
Illustrate the HIV-1 spike protein using a neat diagram.
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4.
How many subunits constitute HIV-1/ENV GP160 trimer?
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5.
Discuss the issues in the development of an effective cholera vaccine.
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6.
Discuss the issues in the development of an effective HIV-1/AIDS vaccine.
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7.
Illustrate the CTB and CTA interface in a cholera toxin.
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8.
Illustrate the CTB interfaces in a cholera toxin.
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9.
Illustrate the GP120/GP40 interface in the HIV-1/ENV protein.
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10.
Discuss HIV-1/ENV GP160 trimer spike structure.
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Kangueane, P., Nilofer, C. (2018). Protein-Protein Interfaces and Diseases. In: Protein-Protein and Domain-Domain Interactions. Springer, Singapore. https://doi.org/10.1007/978-981-10-7347-2_16
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DOI: https://doi.org/10.1007/978-981-10-7347-2_16
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Publisher Name: Springer, Singapore
Print ISBN: 978-981-10-7346-5
Online ISBN: 978-981-10-7347-2
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