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Basic Techniques in Fluorescence and Electron Microscopy

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Cyanidioschyzon merolae

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Abstract

The primitive red alga Cyanidioschyzon merolae is a single cell with a very small size (1.5–2 μm) and has no thick cell wall; therefore, preparation of samples for fluorescence and electron microscopy requires different procedures compared to those for green algae or higher plants. Here, fluorescence microscopy methods, such as cell staining with fluorescent dye and the setup of the immunofluorescence microscope, are described. For electron microscopy, methods for transmission electron microscopy, immunoelectron microscopy, and negative staining methods are described. In addition, scanning electron microscopy methods for visualizing cells or organelles are presented.

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Authors and Affiliations

  1. Department of Chemical and Biological Science, Faculty of Science, Japan Women’s University, 2-8-1 Mejirodai, Bunkyo-ku, Tokyo, 112-8681, Japan

    Haruko Kuroiwa

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  1. Haruko Kuroiwa
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Correspondence to Haruko Kuroiwa .

Editor information

Editors and Affiliations

  1. Faculty of Science Department of Chemical and Biological Sciences, Japan Women’s University, Tokyo, Japan

    Tsuneyoshi Kuroiwa

  2. Department of Cell Genetics, National Institute of Genetics, Shizuoka, Japan

    Shinya Miyagishima

  3. Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Chiba, Japan

    Sachihiro Matsunaga

  4. Department of Life Sciences Graduate School of Arts and Sciences, The University of Tokyo, Tokyo, Japan

    Naoki Sato

  5. Department of Biological Sciences Graduate School of Science, University of Tokyo, Tokyo, Japan

    Hisayoshi Nozaki

  6. Laboratory for Chemistry and Life Science Institute of Innovative Research, Tokyo Institute of Technology, Kanagawa, Japan

    Kan Tanaka

  7. Graduate School of Science and Technology for Innovation Faculty of Science, Department of Biological Science and Chemistry, Yamaguchi University, Yamaguchi, Japan

    Osami Misumi

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Kuroiwa, H. (2017). Basic Techniques in Fluorescence and Electron Microscopy. In: Kuroiwa, T., et al. Cyanidioschyzon merolae. Springer, Singapore. https://doi.org/10.1007/978-981-10-6101-1_8

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