Abstract
Majority of cancer cells up-regulate co-inhibitory molecule PD-L1 which inhibits T cell activation and proliferation, and allows cancers to escape from host immune surveillance, suggesting that it is a potential cancer therapeutic target in drug discovery. To screen the small molecule inhibitors down-regulating the transcriptional activity of PD-L1 promoter, the luciferase reporter expressing vector containing PD-L1 promoter was constructed, then the activity of promoter was detected in the HeLa cells. Moreover, the screening model of small molecule inhibitors suppressing the transcriptional activity of PD-L1 promoter using the luciferase reporter assay system was established, and we identified a small molecule chemical Fludarabine Phosphate which inhibited the activity of PD-L1 promoter with the inhibitory rate of 0.6. Furthermore, Fludarabine Phosphate inhibited the expression of PD-L1 at both mRNA and protein levels in MGC-803 cells. Our study provides a useful tool for screening small molecules efficiently inhibiting PD-L1 expression.
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This research is supported by the program for College students’ innovative entrepreneurial training plan (201510057058).
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Jiang, B. et al. (2018). Construction of the PD-L1 Promoter-Luciferase Reporter Expressing Vector for Small Molecule Inhibitors Screening. In: Liu, H., Song, C., Ram, A. (eds) Advances in Applied Biotechnology. ICAB 2016. Lecture Notes in Electrical Engineering, vol 444. Springer, Singapore. https://doi.org/10.1007/978-981-10-4801-2_72
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DOI: https://doi.org/10.1007/978-981-10-4801-2_72
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