Successful collection of stem cells in one day in the process of autologous stem cell transplantation

Abstract

Autologous stem cell transplantation (ASCT) is an established treatment for patients with hematologic malignancies. The minimum CD34+ cell dose needed to ensure hematopoietic recovery following ASCT is 2x106 cells/kg. The aim of this paper is to prove that absolute number of cells (ANC) CD34+>=20,01x106/L in the peripheral blood is confirmation of successful collection. The study was a retrospective-prospective. We analysed 44 patients Myeloma Multiplex 23(33.3%), Hodgkin lymphoma 29 (42.0%), non-Hodgkin Lymphoma 17 (24.6%) with 69 apheresis conducted in the process of autologous stem cell transplantation at Haematology clinic KCUS in the period from April 2013 to October 2016. Patients were divided into two groups: collection of CD34+<2.0x106/kgand>=2.0x106/kgin one day.Independent variables analyzed were: CD34+cell dose in peripheral blood in one collection, age, gender and diagnosis. The average age of patients was 45 ± 16.03 years. Youngest patient was 18 and the oldest was 67 years. There were 52.3% male patients and 47.7% female.Collection of CD34+ <2.0x106/kg occurred in 20 (27.5%) cases and 49 (72.5%) had CD34+≥ 2.0. In the group with CD34+ ≥2.0x106/kg there were 91.8% with ANC CD34+ ≥20.01 x106 /l in peripheral blood. Only three collections of CD34+ >=2.0x106/kg had values in peripheral blood ANC CD34 + from 15.01 to 20.00x106 /l and only one collection ANC CD34+ between 10.01 and 15.00 x106 /l. Displayed difference is statistically significant p=0.0001. We used the c2 test, Kolmogorov Smirnov and Mann-Whitney U test. P<0.05 was considered significant. Successful collection is guaranteed at ANC CD34+ >= 20.01 x 106/L in the peripheral blood.