Abstract
Bioengineering of synthetic metabolic pathways is a valuable tool for production of useful products and basic understanding of the biological complexity. Thus, we require high-throughput cloning and characterization tools and technologies. Standard cloning techniques have limitation for construct size and slow process. Here, we underline recent advancement and development of high-throughput technologies which can help to accelerate the synthetic biology research. It can be useful for the construction of large gene cassettes for production of drugs, bio-fuels, therapeutics and also rewired the natural systems; and created novel gene networks. In this chapter, we confer the different gene cloning methods for assembly of gene networks and their characterization using recent synthetic biology tools. It can be useful for accelerating the synthetic biology research for human welfare.
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Acknowledgements
Authors wish to thank A.K. Singh, Satya Prakash and Pritee Singh for providing the suggestions, encouragement and fruitful discussion during preparation of this chapter.
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Singh, V., Mani, I., Chaudhary, D. (2015). Advancement of Emerging Tools in Synthetic Biology for the Designing and Characterization of Genetic Circuits. In: Singh, V., Dhar, P. (eds) Systems and Synthetic Biology. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-9514-2_17
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DOI: https://doi.org/10.1007/978-94-017-9514-2_17
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