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MALDI Imaging Mass Spectrometry

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Detection of Chemical, Biological, Radiological and Nuclear Agents for the Prevention of Terrorism

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Abstract

Matrix assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS) is a powerful tool for the in situ analysis of endogenous biomolecules and exogenous pharmaceutical compounds in thin tissue sections. Hundreds to thousands of analytes can be detected and mapped in a single imaging experiment without a priori knowledge of the exact molecules present in the section. Advances in sample preparation and in instrumentation have allowed for MALDI IMS to become a high throughput technique allowing for statistical analysis and diagnostic classification of clinically important specimens. This review discusses the current state of the technology with regards to advances in sample preparation techniques and instrumentation. These include the expansion of the technique to the analysis of formalin-fixed, paraffin embedded tissues as well as the ability to acquire IMS data at the single cell level. Applications to skin cancer, infectious disease, diabetes, and age-related macular degeneration are highlighted.

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Acknowledgements

The authors thank Rossitza Lazova (Yale University Medical School) and Alireza Sepehr (Harvard University Medical School) for contribution and histological evaluation of melanoma samples and Eric Skaar (Vanderbilt University School of Medicine) for collaborative efforts with the S.aureus studies. The work on diabetic nephropathy was carried out by Kerri Grove and Billy Hudson (Vanderbilt University School of Medicine). Studies of AMD were performed by Kevin Schey and David Anderson (Vanderbilt University School of Medicine) in collaboration with Rosalie Crouch (Medical University of South Carolina). Funding from NIH/NCRR 8P41 GM103391 and NIH/NIGMS 5R01 GM58008 are gratefully acknowledged.

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Authors and Affiliations

  1. Mass Spectrometry Research Center and the Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN, USA

    Erin H. Seeley & Richard M. Caprioli

Authors
  1. Erin H. Seeley
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  2. Richard M. Caprioli
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Correspondence to Richard M. Caprioli .

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Editors and Affiliations

  1. Fisheries and Oceans Canada, Science Branch, Special Projects and Memorial University of New Foundland, St. John's, Canada

    Joseph Banoub

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Seeley, E.H., Caprioli, R.M. (2014). MALDI Imaging Mass Spectrometry. In: Banoub, J. (eds) Detection of Chemical, Biological, Radiological and Nuclear Agents for the Prevention of Terrorism. NATO Science for Peace and Security Series A: Chemistry and Biology. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-9238-7_7

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