Abstract
In the last several years more direct evidences have been obtained that individual lipids are associated with PSI or PSII structures (Siegenthaler 1982). In a previous paper (Krupa 1982) it was shown that hydrolysis of galactolipids by bean GL causes the release of significant amounts of plastocyanin as a parallel effect due to breakdown of thylakoid membrane lipids. It was also shown (Krupa 1983) that thylakoid membranes treated with bean GL or PLA2 demonstrated marked changes in their polypeptide patterns when separated on SDS-PAGE, suggesting some relationships between the particular groups of thylakoid lipids and polypeptides originating from chlf-protein complexes. The aim of investigations presented here was to study the changes in chlf-protein complexes of bean GL and PLA2, PLC and PLD-treated thylakoid membranes.
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Abbreviations
- chlf:
-
chlorophyll
- CPla, CP1:
-
chlf a-protein complexes of PSI
- CPa:
-
chlf a-protein complex of PSII
- DGDG:
-
digalactosyldiacylglycerol
- FC:
-
free chlf
- GL:
-
galactolipase
- Co :
-
control thylakoids (nonincubated)
- CI :
-
thylakoids incubated without enzyme
- LHPC1–3 :
-
light-harvesting chlf a/b protein complexes
- MGDG:
-
monogalactosyldiacylglycerol
- PAGE:
-
polyacrylamide gel electrophoresis
- PC:
-
phosphatidylcholine
- PG:
-
phosphatidylglycerol
- PLA2c :
-
phospholipase A2 from Crotalus terr. terr.
- PLA2p :
-
phospholipase A2 from porcine pancreas
- PLC:
-
phospholipase C
- PLD:
-
phospholipase D
- SDS:
-
sodium dodecyl sulphate
- SQDG:
-
sulfoquinovosyldiacylglycerol
References
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© 1984 Springer Science+Business Media Dordrecht
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Krupa, Z. (1984). Changes in Chlorophyll-protein Complexes of Bean Galactolipase-or Phospholipase A2, C and D-Treated Thylakoid Membranes. In: Sybesma, C. (eds) Advances in Photosynthesis Research. Advances in Agricultural Biotechnology, vol 3. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-4973-2_26
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DOI: https://doi.org/10.1007/978-94-017-4973-2_26
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