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Some Characteristics of a Ribulose-1,5-bisphosphate Carboxylase Deficient Green Tobacco Mutant in Cell Suspension Cultures

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Advances in Photosynthesis Research

Part of the book series: Advances in Agricultural Biotechnology ((AABI,volume 3))

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Abstract

An unusual viridis SP25 mutant of Nicotiana tabacum was selected by a mutagenic treatment of seeds with ethyl methyl sulfonate (Dulieu, 1981). The green leaf of SP25 mutant showed no variation among the plastid membranous polypeptides but no Fraction I (RUBPC) could be detected by gel electrophoresis technique in the soluble protein phase. The present report describes growth characteristics, pigment content, plastid ultrastructure and photochemical activity of in vitro cell suspension cultures derived from homogeneous leaf tissue of SP25 mutant. The study of the enzymatic reduction of NO 3 related to cellular reduction power was also performed in different growth conditions.

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References

  • Blondel AM and Blanc D (1975) Mise au point d’une methode de mesure in vivo de l’activité nitrate réductase, Ann. agr. 26, 3, 309–322.

    CAS  Google Scholar 

  • Dulieu H (1981) L’induction de variabilité génétique dans le plastome. Recherche de protéines modifiées dans les chloroplastes. In Proceeding of Intern. Atomic Energy Agency, 251, 67–80.

    Google Scholar 

  • Evans H.J. and Nason A (1953) Pyridine nucleotide-nitrate reductase from extracts of higher plants. Plant Physiol. 28, 233–254.

    Article  PubMed  CAS  Google Scholar 

  • Hewitt EJ, Hucklesby DP, Mavin AF, Notton BA and Rucklidge GJ (1979) In Nitrogen Assimilation of plants (EJ Hewitt and CV Cutting, eds), pp 255–287, Academic Press N.Y.

    Google Scholar 

  • Maurino SG, Varga MA, Aparicio PJ and Maldonado JM (1983) Blue-light reactivation of spinach nitrate réductase inactivated by acetylene or cyanide. Effects of flavins and oxygen, Physiol. Plant. 57, 411–416.

    Article  CAS  Google Scholar 

  • Miginiac-Maslow M, Mathieu Y, Nato A and Hoarau A (1981) Contribution of photosynthesis to the growth and differentiation of cultured tobacco cells. The regulatory role of inorganic phosphate. In Proceeding Photosynthesis V. Chloroplast development, edited by G. Akoyunoglou, 977-984.

    Google Scholar 

  • Nato A, Bazetoux S and Mathieu Y (1977) Photosynthetic capacities and growth characteristics of Nicotiana tabacum (cv Xanthi) cell suspension cultures, Physiol. Plant. 41, 116–123.

    CAS  Google Scholar 

  • Nato A, Brangeon J and Dulieu H (1981) Nuclear gene mutation effects on the plastid structure and RUBPCase properties in Nicotiana tabaoum (cv Xa nthi), In Proceeding Photosynthesis V. Chloroplast development edited by G. Akoyunoglou, 821-830.

    Google Scholar 

  • Nato A, Hoarau J and Bourdu R (1983) Effect of light intensity during growth on physiological and photosynthetic characteristics of cell suspension cultures of Nicotiana tabacum, Biol. Cell. 47, 213–218.

    CAS  Google Scholar 

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© 1984 Springer Science+Business Media Dordrecht

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Aime, N., Jackson, H., Rita, B., Hubert, D. (1984). Some Characteristics of a Ribulose-1,5-bisphosphate Carboxylase Deficient Green Tobacco Mutant in Cell Suspension Cultures. In: Sybesma, C. (eds) Advances in Photosynthesis Research. Advances in Agricultural Biotechnology, vol 3. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-4973-2_181

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  • DOI: https://doi.org/10.1007/978-94-017-4973-2_181

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-90-247-2944-9

  • Online ISBN: 978-94-017-4973-2

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