Skip to main content

Light Scattering, Fluorescence Yield and Membrane Stacking Changes due to Divalent Cation Removal from Stacked Thylakoids

  • Chapter
Advances in Photosynthesis Research

Part of the book series: Advances in Agricultural Biotechnology ((AABI,volume 3))

  • 12 Accesses

Abstract

Changes in thylakoid stacking, chlorophyll fluorescence yield and light scattering upon varying the cation composition of a broken chloroplast suspension have been widely reported. Contrasting results have been presented about a strict correlation between changes in light scattering or thylakoid stacking and changes in fluorescence yield (Murakami, Packer, 1971; Murata, 1971; Gross, Hess, 1974; Krause, 1974; Argyroudi-Akoyunoglou, Akoyunoglou, 1977; Wollman, Diner, 1980), whereas the changes in thylakoid stacking and light scattering have been proposed to be closely related (Wollman, Diner, 1980). In this work we reinvestigated the possible correlation between these phenomena by studying the kinetics of their decrease upon EDTA addition to Mg-ion stacked chloroplasts.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 74.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Argyroudi-Akoyunoglou JH and Akoyunoglou G (1977) Correlation between cation-induced formation of heavy subchloroplast fraction and cation-induced increase in chlorophyll a fluorescence yield in tricine-washed chloroplasts, Arch. Biochem. Biophys. 179, 370–377.

    Article  PubMed  CAS  Google Scholar 

  • Gross EL and Hess SC (1974) Correlation between calcium ion binding to chloroplast membranes and divalent cation-induced structural changes and changes in chlorophyll a fluorescence, Biochim. Biophys. Acta 339, 334–346.

    Article  PubMed  CAS  Google Scholar 

  • Gross EL and Prasher SH (1974) Correlation between cation-induced decreases in chlorophyll a fluorescence and chloroplast structural changes, Arch. Biochem. Biophys. 164, 460–468.

    Article  PubMed  CAS  Google Scholar 

  • Jennings RC, Garlaschi FM and Gerola PD (1983) A study on the lateral distribution of the plastoquinone pool with respect to photosystem II in stacked and unstacked spinach chloroplasts, Biochim. Biophys. Acta 722, 144–149.

    Article  CAS  Google Scholar 

  • Jennings RC, Gerola PD, Garlaschi FM and Forti G (1980) Studies on the fractionation by digitonin of chloroplast membranes, FEBS Lett. 115, 39–42.

    Article  CAS  Google Scholar 

  • Krause GH (1974) Light-induced movement of metal cations in chloroplasts, evidence from chlorophyll fluorescence and light scattering. In: Avron M, ed., Proceedings of the third international congress on photosynthesis, pp. 1021–1030. Amsterdam: Elsevier.

    Google Scholar 

  • Murakami S and Packer L (1971) The role of cations in the organizatin of chloroplast membranes, Arch. Biochem. Biophys. 146, 337–347.

    Article  PubMed  CAS  Google Scholar 

  • Murata N (1971) Control of excitation transfer in photosynthesis. V. Correlation of membrane structure to regulation of excitation transfer between two pigment systems in isolated spinach chloroplasts, Biochim. Biophys. Acta 245, 365–372.

    Article  PubMed  CAS  Google Scholar 

  • Wollman FA and Diner BA (1980) Cation control of fluorescence emission, light scattering and membrane stacking in pigment mutants of Chlamydomonas reinhardi, Arch. Biochem. Biophys. 201, 646–659.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1984 Springer Science+Business Media Dordrecht

About this chapter

Cite this chapter

Garlaschi, F.M., Gerola, P.D., Jennings, R.C., Forti, G. (1984). Light Scattering, Fluorescence Yield and Membrane Stacking Changes due to Divalent Cation Removal from Stacked Thylakoids. In: Sybesma, C. (eds) Advances in Photosynthesis Research. Advances in Agricultural Biotechnology, vol 3. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-4973-2_18

Download citation

  • DOI: https://doi.org/10.1007/978-94-017-4973-2_18

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-90-247-2944-9

  • Online ISBN: 978-94-017-4973-2

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics