Abstract
Advances in the genetic mapping of wheat, the molecular interpretation of flour processing traits and large-scale sequencing of genes expressed in endosperm tissue are currently converging to define the genes that under-pin key quality traits. In order to achieve this, accurate definitions of a phenotype such as dough extensibility are essential and in this paper a molecular /genetic analysis of this trait is presented. Studies carried out on the small-scale Mixograph have provided the data to indicate that the mixing action in this system develops the dough through multiple elongate-rupture-relax cycles of the flour/water mixture. The measurement can be used to define a variable we refer to as “M-extensibility”, a measure very closely related to the traditional extensibility measurement and most likely a sub-component of this “classical” assessment. Analyses based on molecular genetic maps have shown that both LMW and HMW glutenin loci most likely account for significant variation in M-extensibility. In addition, it is evident that genes on chromosome 2 also contribute and work is in progress to characterize these genes. The possibility will be discussed that new seed storage protein genes being discovered from the analysis of 5,000 cDNA’s from endosperm tissue (8–12 days post anthesis) have a role to play in M-extensibility.
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References
Bechtel D.B., Zayas, I., Kaleikau, L., and Pomeranz, Y. (1990): Size-distribution of
wheat starch granules during endosperm development. Cereal Chemistry 67, 59–63.
Belton, PS. (1999): On the elasticity of wheat gluten J. Cereal Sci. 29, 103–107.
Briarty, LG., Hughes, CE., Evers, AD. (1979): The developing endosperm — a stereological analysis. Annals of Botany 44, 641–658.
Clarke, BC., Hobbs, M., Skylas, D., Appels, R. (2000): Genes active in developing wheat endosperm. Functional and Integrative Genomics 1 in press.
Curcio, J. A and Petty, C. C. (1951): The near infrared absorption spectrum of liquid water. Journal of the Optical Society of America 41, 302–304.
Gras, PW., Carpenter, HC., Anderssen, RS. (2000): Modelling the developmental rheology of wheat flour doughs using extension tests. J. Cer. Sci. 31, 1–13.
Li, A., Walker, CE., (1992): Dough temperature changes during mixing in a Mixograph. Cereal Chemistry 69, 681–683.
McGuire, PE., Qualset, CO. (1997): Progress in genome mapping of wheat and related species: Joint Proc. of the 5’11 and 6th public workshops of the International Triticeae Mapping Initiative. Report No. 18, University of California Resource Conservation Program, Davis CA, USA.
Nelson, JC. (1997): QGENE: Software for marker-based genomic analysis and breeding. Mol. Breeding 3, 239–245.
Skerritt, J.H., Hac, L., and Bekes, F. (1998): Depolymerisation of the gluten macropolymer during dough mixing: I. Changes of levels, molecular weight distribution and overall composition., Cereal Chem. 76, 395–401.
Shewry, P.R., Miles, M.J. and Tatham, A.S. (1994): The prolamin storage proteins of wheat and related cereals. Prog. Biophys. Molec. Biol. 61, 37–59.
Shewry, PR. (1996): Assembly and post-translational modification of wheat gluten proteins. In Proc. 6th International Gluten Workshop (ed C. Wrigley ), pp 121–128.
Simmonds, D.H. and O’Brien, T.P. (1981): Morphological and biochemical development of the wheat endosperm. In: Y. Pomeranz (Eds). Advances in Cereal Science and Technology. American Association of Cereal Chemists. 5–70.
Tamas, T., Bekes, F., Greenfield, J., Tatham, A., Gras, P.W., Shewry, P., Appels, R. (1996): Heterologous expression and dough mixing studies on genetically modified C-hordeins. J. Cereal Sci. 27, 15–22.
Wesley, I.J, Appels, R., Blakeney, A.B. (2000): Applications of dynamic NIR spectroscopy to cereal science. RACI conference, in press
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Appels, R., Gras, P., Clarke, B.C., Anderssen, R., Wesley, I., Békés, F. (2001). Molecular Genetic Studies on Processing Traits of Wheat Flour. In: Bedö, Z., Láng, L. (eds) Wheat in a Global Environment. Developments in Plant Breeding, vol 9. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-3674-9_6
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DOI: https://doi.org/10.1007/978-94-017-3674-9_6
Publisher Name: Springer, Dordrecht
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