Abstract
We report the design, fabrication, and performance characterization of plastic microfluidic systems for conducting (1) highly parallel enzyme-based assays using sub-microliter reagent volumes and (2) high-speed, high-resolution separation of double- and single-stranded DNA fragments for applications including genotyping and sequencing. To reduce reagent consumption, we have developed a zero-dead-volume dispensing technology for the simultaneous parallel pickup of nL volumes of 96 different reagents from a “library plate” and rapid delivery to a microfluidic assay card. We also demonstrate the capability to perform unlidded 200nL assays with one hour incubations. Recent DNA sequencing results are reported: up to 700 single-stranded DNA fragments are detected with single-base resolution in 40 minutes using a plastic microfluidic device with a 4-color detection system.
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© 2000 Springer Science+Business Media Dordrecht
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Boone, T.D. et al. (2000). Sub-Microliter Assays and DNA Analysis on Plastic Microfluidics. In: van den Berg, A., Olthuis, W., Bergveld, P. (eds) Micro Total Analysis Systems 2000. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-2264-3_127
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DOI: https://doi.org/10.1007/978-94-017-2264-3_127
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-481-5496-8
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