Abstract
Disease-preventing activities of plant products were investigated. Augmentation of vascular smooth muscle cell (SMC) proliferation is known to be a typical phenomenon of atherosclerosis. Genistein and (-)-epigallocatechin gallate impaired proliferation of cultured aortic SMC A-10 cells concentration-dependently. Also, high concentrations of emodin apparently inhibited proliferation. Conversely, quercetin impaired proliferation moderately. The inhibitory effects of the chemicals on mitochondrial enzyme activity were temperate in A-10 cells. Cell death rates increased according to the concentrations of the chemicals. However, their rates were much smaller than the inhibition rates of cell proliferation, indicating that the chemicals retarded aortic SMC proliferation. Rubratoxin B, a potent hepatotoxic mycotoxin, is considered to retard cell proliferation and induce apoptosis and corresponding internucleosomal fragmentation (DNA ladder). Genistein and emodin significantly impaired rubratoxin B-caused retardation of proliferation and DNA ladder in HL60 cells. Therefore, genistein and emodin may prevent rubratoxin B-caused hepatic injury. In conclusion, plant products used in this study could effectively prevent atherosclerosis and chemical-caused hepatic injury.
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© 2002 Springer Science+Business Media Dordrecht
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Nagashima, H., Nakamura, K., Goto, T. (2002). Disease-Preventing Activities of Plant Products. In: Shirahata, S., Teruya, K., Katakura, Y. (eds) Animal Cell Technology: Basic & Applied Aspects. Animal Cell Technology: Basic & Applied Aspects, vol 12. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0728-2_79
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DOI: https://doi.org/10.1007/978-94-017-0728-2_79
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