Abstract
In dietary shellfishes, we found that the hot water extract of hard clam (Meretrix lusoria) showed immune bioactivity remarkably. It was clarified that the principle induced cell proliferation of mouse spleen cells, T cell lines, macrophages and human peripheral blood mononuclear cells, and also enhanced immunoglobulin secretion in human hybridoma cell lines. In this report, we showed the mechanisms for antitumor effects of the principal S75 II. Cell viability was quantified by MU assay, and apoptosis was determined by both PI staining and DNA fragmentation. Tumor cells treated with S75 II (10 µg/ml for 24h) exhibited patterns of apoptotic cell death. Tumor cells pretreated with swainsonine, a α -mannosidase II inhibitor, the apoptosis-inducingg activity was blocked significantly. In the presence of caspase inhibitor z-VAD-fink partially rescue cell viability, implying that apoptosis occurrence via the activation of caspase family. Our results suggest that the principle of Meretrix lusoria may be a novel sialic acid-binding lectin, and its antitumor effects was probably through caspase related apoptosis mediated by cell membrane glycosyl-moiety interaction.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
Enari, M., Talanian, R. V., Wong, W. W., Nagata, S. (1996) Sequential activation of ICE-like and CPP32-like protease during Fas-mediated apoptosis. Nature 380: 723–726.
Kong, Z. L., Miwa, M., Murakami, H. and Shinohara, K. (1990) Establishment of a macrophagelike cell line derived from U937, human histiocytic lymphoma, grown serum-freeJn Vitro Cell. Dey. Biol. 26: 949–954.
Mayne, G. C. and Murrary, A. W. (1998) Evidence that protein kinase Ce mediates phorbol ester inhibition of calphostin C- and tumor necrosis factor-a-induced apoptosis in U937 histiocytic lymphoma cells. J. Bio. Chem. 273 (37): 24115–24121.
Shinohara, K., Fukushima, T., Suzuki, M., Tsutsumi, M., Kobori, M. and Kong, Z. L. (1993) Effect of some constituents of chicken egg yolk lipoprotein on the growth and IgM production of human-human hybridoma cells and other human-derived cells. Cytotechnology. 11 (2): 149–54.
Kong, Z. L., Chiang, L. C., Fang, F., Shinohara, K. and Pan, R (1997) Immune bioactivity in shellfish toward serum-free cultured human cell lines. Biosci. Biotech. Biochem. 61 (1) 24–28.
Itoh, H., No a, H., Amano, H., Zhuaug, C., Mizuno, T. and Ito, H. (1993) Antitumor activity and immunological properties of marine algal polysaccharides, especially fucoidan, prepared from Sargassum thunbergii of Phaeophyceae. Anticancer Res. 13 (6A): 2045–52.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2002 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Kong, ZL., Hwang, CC., Lai, CW. (2002). Apoptosis and Receptor-Mediated Signal Regulation of Cultured Tumors by Glycoprotein from Meretrix lusoria . In: Shirahata, S., Teruya, K., Katakura, Y. (eds) Animal Cell Technology: Basic & Applied Aspects. Animal Cell Technology: Basic & Applied Aspects, vol 12. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0728-2_77
Download citation
DOI: https://doi.org/10.1007/978-94-017-0728-2_77
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-481-5934-5
Online ISBN: 978-94-017-0728-2
eBook Packages: Springer Book Archive