Abstract
In a previous experiment (Isoda et al., 2001), we showed that the tight-junctional (TJ) permeability increase in Caco-2 cells during capsaicin exposure was through binding of the capsaicin molecule to a capsaicin receptor-like protein. In the present study, we examined how actin, which modulates TJ permeability, is influenced by capsaicin. We showed that after treatment of the Caco-2 cells with capsaicin, the volume of F-actin decreased. Moreover, we also examined protein kinase C (PKC) and heat shock protein 47 (HSP47), which act as probable second messengers in causing TJ permeability increase. We showed that after capsaicin treatment, HSP47 was activated. However, PKC activity was the same in both control and treatment setups. These results suggest that, while PKC is not involved, it is highly possible that HSP47 plays a role in TJ permeability increase in intestinal Caco-2 cells exposed to capsaicin.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Diange L, Razzaque MS, Cheng M and Taguchi T (2002) The renal expression of Heat Shock Protein 47 and collagen in acute and chronic experimental diabetes in rat. Histochemical Journal 33: 621–628.
Erika Jensen-Jarolim, Leszek Gajdzik, Ines Haberl, Dietrich Kraft, Otto Scheiner and JUrg Graf (1997) Human Nutrition and Metabolism. J.Nutr 6: 577–581.
Hashimoto K, Kawagishi H, Nakayama T and Shimizu M (1997) Effect of capsianoside, a diterpene glycoside, on tight-junctional permeability. Biochimica et Biophysica Acta 1323: 281–290.
Holmes KC, Popp D, Gebhard W and Kabsch W (1990) Atomic model of the actin filament. Nature 347: 44–49.
Isoda H, Han JK, Tominaga M and Maekawa T (2001) Effect of capsaicin on human intestinal cell line Caco-2. Cytotechnology 36: 155–161.
Michael J. Caterina, Mark A Schumacher, Makoto Tominaga, Tobias A. Rosen, Jon D. Levine and David Julius (1997) The capsaicin receptor: a heat-activated ion channel in the pain pathway. NATURE 389: 816–824.
Razzaque M S, Shimokawa I, Nazneen A, Higami Y and Taguchi T (1998) Age-related nephropathy in the Fischer 344 rat is associated with overexpression of collagens and collagen-binding heat shock protein 47. Cell and Tissue Research 293: 471–478.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2003 Springer Science+Business Media Dordrecht
About this paper
Cite this paper
Han, J.K., Isoda, H., Tominaga, M., Maekawa, T. (2003). Analysis of the Mechanism of the Tight-Junctional Permeability Increase by Capsaicin Treatment on the Intestinal Caco-2 Cell. In: Yagasaki, K., Miura, Y., Hatori, M., Nomura, Y. (eds) Animal Cell Technology: Basic & Applied Aspects. Animal Cell Technology: Basic & Applied Aspects, vol 13. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0726-8_78
Download citation
DOI: https://doi.org/10.1007/978-94-017-0726-8_78
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-481-6557-5
Online ISBN: 978-94-017-0726-8
eBook Packages: Springer Book Archive