Abstract
Changes in content, constituents and distribution of constitutive and excreted sugars of Spirulina (Arthrospira) maxima in nitrogen or sulfur-limited batch cultures were investigated in this study. The changes were influenced by both age of the culture and availability of nitrogen or sulfur in the medium. Increases in percentage of the constitutive sugars (PSC) took place at the beginning of the decelerating phase. The increase was mainly due to the accumulation of internal soluble polysaccharides (SIPS). The maximal concentration of PSC per unit volume of reactor was obtained at the beginning of the death phase, at which time cells from nitrogen-limited batch cultures contained about 66% of sugars. SIPS representing 76% of PSC consisted of mainly glucose. The monosaccharide of cell wall polysaccharides (CWPS) was also glucose. Sugars from the external layers (PSEL) or from culture medium (PSCM) contained a mixture of monosaccharides. Selective fractionation of PSCM, PSEL, SIPS and CWPS was obtained by a sequential extraction procedure.
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References
Beardall, J., Griffiths, H. and Raven, J. A. (1982) Carbon isotope discrimination and the CO2 accumulating mechanism in Chlorella emersonii. J. Exp. Bot. 33, 729–737.
Beardall, J., Boberts, S. and Millhouse, J. (1991) Effects on nitrogen limitation on uptake of inorganic carbon and specific activity of ribulose-1,5-bisphophate carboxylase/oxgenase in green microalgae. Can. J. Bot. 69, 1146–1150.
Behrens, P.W., Sicotte, V.J. and Delente, J. (1994) Microalgae as a source of stable isotopically labeled compounds. J. Appl. Phycol. 6, 113–121.
Bertocchi, C., Navarini, L. and Cesàro, A. (1990) Polysaccharides from cyanobacteria. Carbohydrate Polymers 12, 127–153.
Black, G.E. and Fox, A. (1996) Recent progress in the analysis of sugar monomers from complex matrices using chromatography in conjunction with mass spectrometry or stand-alone tandem mass spectrometry. J. Chromatogr. A 720, 52–59.
Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantites of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248–254.
Chambers, R. E. and Clamp, J.R. (1971) An assessment of nnethanolysis and other factors used in the analysis of carbohydrate-containing materials. J. Biochem. 125, 1009–1018.
Clamp, J.R., Bhatti, T. and Chambers, R.E. (1972) The determination of carbohydrate in biological materials by gas-liquid chromatography, in Methods of Biochemical Analysis, vol. 19, Academic Press, New York, pp. 229–344.
de Vasconcelos, L. and Fay, P. (1974) Nitrogent metabolism and ultrastructure in Anabaena cylindrica I. The effect of nitrogen starvation. Arch. Microbiol. 96, 271–279.
Filalimouhim, R., Cornet, J.F., Fontane, T., Fournet, B. and Dubert, G. (1993) Production, isolation and preliminary characterization of the exopolysaccharide of the cyanobacterium Spirulina platensis. Biotech. Lett. 15, 567–572.
Garbacki, N., Gloaguen, V., Damas, J., Hoffmann, L., Tits, M. and Angenot, L. (2000) Inhibition of croton oilinduced edema in mice ear skin by capsular polysaccharides from cyanobacteria Naunyn Schmiedeberg’s Arch. Pharmacol. 361, 460–464.
Ghoos, Y., Geypens, B., Maes, B., Hiele, M. and Rutgeerts, P. (1994) 13CO2-breath tests as diagnostic tools in gastroenterology, in S. Goldstein, P. Louvet and E. Soulié (eds), Less Isotopes Stables Applications Production, l’ Institut National des Sciences et Techniques Nucléaires, Centred’ Études de Saclay, pp. 23–30.
Goodwin, T.W. (1974) Carotenoids and biliproteins, in W.D.P. Stewart (ed.) Algal Physiology and Biochemistry, Blackwell Scientific Publications, New York, pp. 176–200.
Ha, Y.W., Dyck, L.A. and Thomas, R.L. (1988) Hydrocolloids from the freshwater microalgae, Palmella texensis and Cosmarium turpinii. J. Food Sci. 53, 841–845.
Herbert, D., Phipps, P.J. and Strange, R.E. (1971) Chemical analysis of microbial cells, in J. R. Norris and D. Ribbons (eds.), Methods in Microbiology, vol. Sb, Academic Press, New York, pp. 210–336.
Kaplan, D., Richmond, A.E., Dubinsky, Z. and Aaronson, S. (1986) Algal nutrition, in A. Richmod (ed.), Handbook ofMicroalgal Mass Culture, CRC Press, New York, pp. 147–198.
Levert, J.M. and Xia, J. L. (2001) Modeling the growth curve for Spirulina (Arthrospira) maxima, a versatile microalga for producing uniformly labelled compounds with stable isotopes. J. Appl. Phycol. (in press).
Melta, V.B. and Vaidya, B.S. (1978) Cellular and extracellular polysaccharides of the blue-green alga Nostoc. J. Exp. Bot. 29, 1423–1430.
Meeks, J.C. (1974) Chlorophylls, in W.D.P. Stewart (ed.) Algal Physiology and Biochemistry, Blackwell Scientific Publications, New York, pp. 161–171.
Merril, C. R. (1990) Gel staining techniques, in M.P. Deutscher (ed.), Methods in Enzymology, vol. 182, Academic Press, New York, pp. 477–488.
Nicolaus, B., Panico, A. and Lama, L. (1999) Chemical composition and production of exopolysaccharides from representative members of heterocystous and non-heterocystous cyanobacteria. Phytochem. 52, 639–647.
O’Kelley, J.C. (1974) Inorganic nutrients, in W.D.P. Stewart (ed.) Algal Physiology and Biochemistry, Blackwell Scientific Publications, New York, pp. 610–635.
Painter, T.J. (1983) Algal polysaccharides, in G.O. Aspinall (ed.), The Polysaccharides, vol. 2, Academic Press, New York, pp.195–285.
Raven, J.A. (1974) Carbon dioxide fixition, in W.D.P. Stewart (ed.) Algal Physiology and Biochemistry, Blackwell Scientific Publications, New York, pp. 443–448.
Rawn, J.D. (1983) Biochemistry, Happer & Row Publishers, New York.
Rippka, R., Deruelles, J., Waterbury, J.B., Herdman, M. and Stanier, R.Y. (1979) Generic assignments, strain histories and properties of pure cultures of cyanobacteria. J. Gen. Microbiol. 111, 1–61.
Tease, B.E. and Walker, R.W. (1987) Comparative composition of the sheath of the cyanobacterium Gloeothece ATCC 27152 cultured with and without combined nitrogen. J. Gen. Microbiol. 133, 3331–3339.
van Eykelenburg, C. (1980) Ecophysiological studies on Spirulina platensis effect of temperature, light intensity and nitrate concentration on growth and ultrastructure. Antonie van Leeuwenhoek 46, 113–127.
Vonshak, A., Abeliovich, A., Boussiba, S. and Richmond, A. (1982) Production of Spirulina biomass: Effects of environmental factors and population density. Biomass 2, 175.
Wu, J., Zhang, C.W. and Liu, Y.F. (1999) Isolation, purification and immunological activities of extracellular polysaccharide EP II from Spirulina maxima. Med. Biotech. 6, 99–102.
Xia, J.L. (1999) Contribution To The Study of Photosynthesis of Sugars By Spirulina maxima, Ph.D. Thesis, Faculté Polytechnique de Mons, Mons, Belgium.
Zarrouk, C. (1966) Contribution à l’ étude d’ une cyanobactérie: Influence de divers facteurs physiques et chimiques sur la croissance et la photosynthèse de Spirulina maxima (Setchell et Gardner) Geitler. Thèse, Univ. Paris, France.
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Xia, J.L., Nie, Z.Y., Levert, J.M. (2001). Changes in Content, Constituents and Distribution of Constitutive and Excreted Sugars of Spirulina (Arthrospira) Maxima in Nutrient-Limited Batch Cultures. In: Chen, F., Jiang, Y. (eds) Algae and their Biotechnological Potential. Springer, Dordrecht. https://doi.org/10.1007/978-94-015-9835-4_10
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DOI: https://doi.org/10.1007/978-94-015-9835-4_10
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