Summary
The main purpose of this study was to use the anther culture system to perform in vitro selection for tolerance to low non-freezing temperatures in potato. Anther cultures were incubated at various temperatures (10, 15, 20, 25, 30 °C) for induction of embryos and calli followed by plant regeneration. Anther-derived tuber-bearing clones from clone 73 were analysed for cold tolerance to investigate whether the incubation temperature caused a selection. The criteria for establishing the level of low temperature tolerance included chlorophyll fluorescence, biomass production, and the degree of chlorosis. Although, a correlation between temperature tolerance of the anther-derived clones and the in vitro incubation temperature could not be found, significant differences in tolerance could be identified between the anther-derived clones. Furthermore, a significant correlation was found between the temperature-related embryogenic potential (embryo or calli production at 10 °C/embryo or calli production at the optimal temperature) of 6 tetraploids and their low temperature tolerance. This indicates a relationship between gene-expression of the embryogenic microspore and the sporophyte. Accordingly, even though no selection effects could be found in this particular experiment, prerequisites for in vitro selection seem to be present.
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© 1997 Springer Science+Business Media Dordrecht
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Calleberg, E.K., Merker, A. (1997). Selection for low temperature tolerance in potato through anther culture. In: Tigerstedt, P.M.A. (eds) Adaptation in Plant Breeding. Developments in Plant Breeding, vol 4. Springer, Dordrecht. https://doi.org/10.1007/978-94-015-8806-5_6
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DOI: https://doi.org/10.1007/978-94-015-8806-5_6
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