Abstract
There are now more than 100 different proteins known to be attached to the cell surface of animal or yeast cells by a glycosylphosphatidylinositol(GPI) anchor [1]. The structure of these anchoring units (Fig 1) is relatively highly conserved, although additional sugar moieties are sometimes attached to the basic oligoglycan backbone, and the diacylglycerol lipid unit is in certain molecules replaced by an alkyl,acyl-glycerol or a ceramide structure. Among the more familiar proteins now known to be GPI-anchored are alkaline phosphatase, acetylcholinesterase, and the variable surface glycoprotein coating the surface of trypanosomes. Surprisingly, there have been no reports of GPI-anchored proteins in plants. Because my laboratory has an ongoing project to study the GPI-anchored proteins of the protozoan Tetrahymena, we have been able to apply this technology in a search for these proteins in higher plants.
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References
Low, MG. The glycosyl-phosphatidylinositol anchor of membrane proteins. Biochim B i ophys Acta 1989; 988: 427–454.
Bieleski, RL. Development of an externally-located alkaline phosphatase as a response to phosphorus deficiency. In: Bieleski,R L, Ferguson, AR, Cresswell, MM, editors. Mechanisms of regulation of plant growth. Wellington, Roy Soc NZ, 1974: 165–170.
Malik, A-S, Low, MG. Conversion of human placental alkaline phosphatase from a high Mr form to a low Mr during butanol extraction: an investigation of the role of endogenous phosphoinositide-specific phospholipases. Biochem J 1986; 240:519–527
Wong, YW, Low, MG. Phospholipase resistance of the glycosyl-phosphatidylinositol membrane anchor on human alkaline phosphatase. Clin Chem 1992; 38: 2517–2525.
Ryals, PE, Pak, Y, Thompson, GA, Jr. Phosphatidylinositol-linked glycans and phosphatidylinositol-anchored proteins of Tetrahymena mimbres . J Biol Chem; 1992 266: 15048–15053.
Pak, Y, Ryals, PE, Thompson, GA, Jr. Phosphatidylinositol glycan formation and utilization by the ciliate Tetrahymena mimbres. J Biol Chem; 1992 266: 1 5054 1 5059.
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© 1995 Springer Science+Business Media Dordrecht
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Thompson, G.A., Morita, N., Okuyama, H., Kim, Y., Ko, YG., Hung, CY. (1995). On The Presence of Glycosylphosphatidylinositol-Anchored Proteins in Plants. In: Kader, JC., Mazliak, P. (eds) Plant Lipid Metabolism. Springer, Dordrecht. https://doi.org/10.1007/978-94-015-8394-7_62
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DOI: https://doi.org/10.1007/978-94-015-8394-7_62
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