Anatomy of Adventitious Root Formation in Stem Cuttings of the Easy-to-Root (GemlİK) and Hard-to-Root (Domat) Olive Cultivars

Abstract

The semi-hardwood cuttings of one of the most important black and green olive cultivars of Turkey, Gemlik and Domat, were treated with IBA or by wounding or by wounding+IBA and placed for rooting into Shaded Polyethylene Tunnel (SPT) for 90 days. At 15-day intervals, samples were collected from SPT and examined for morphological variations in the cuttings during rooting. Anatomical studies done in which slides of transverse and longitudinal sections of tissue taken from 1 cm above the site of the cutting. Samples were collected every 15 days during rooting. No morphological differences related with rooting were observed in the histological studies between sample intervals. It was also observed that the sclerenchyma does not appear to be the main source of difficulties in rooting of the Domat cultivar. Increases in metabolic activity may cause callus formation in preference to root formation. The effect of wounding alone did not improve rooting, but did increase the effects of IBA. Also no correlation was found for root initiation and wounding in cuttings. It was observed that in olive cuttings, adventitious roots were seen to emerge from the cambial zone (cambium, ray or undifferentiated secondary xylem cells).