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Identification of Genetic Polymorphism of Poplar (Populus sp.) by Dna Marker Technology

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Progress in Botanical Research

Abstract

In order to identify new Populus lines, STS (Sequence Tagged Site) and RAPD (Random Amplified Polymorphic DNA) marker analysis were performed on eight Populus deltoides x Populus deltoides hybrids and Populus deltoiudes (77/51) which was used as control. For RAPD analysis STS primers were used in single forms. Four of the five STS primers and seven of ten RAPD markers produced amplified PCR (Polymerase Chain Reaction) bands. Polymorphism values were found to be between 7–70%. As a result, it is concluded that STS marker analysis, just like RAPD can also be utilized in selecting and identifying the high-quality poplar lines for paper and pulp industry.

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References

  • Bradshaw Jr., H.D., Villar, M., Watson, B.D., Otto, K.G., Stewart, S., and Stettier, R.F. (1994) Molecular genetics of growt and development in? opulus. II. Agenetic linkage map of a, hybrid poplar composed of RFLP, STS and markers Theor. Appl. Genet. 89, 167–178.

    CAS  Google Scholar 

  • Bradshaw, H.D., Stettier, R.F. (1993) Molecular genetics of growth and development in Populus. I.Triploidy in hybrid poplars. Theor Appl Genet 86, 310–307.

    Article  Google Scholar 

  • Burr, B., Burr, F.A., Thompson, K.H., Albertson, M.C., and Stuber, C.W. (1988) Gene mapping with recombinant inbreds in maize, Genetics 118, 519–526.

    PubMed  CAS  Google Scholar 

  • Dhillon, S.S., DNA in tree species. In: Bonga, J.M., Durzan, D., (eds) (1997) Cell and tissue culturel in foresty, Martinus Nijhoff Pub, Dordrecht, The Netherlands, 1, 298–313.

    Google Scholar 

  • Kesselli, R. V., Paran, I., Michelmore, R. W. (1992) Efficient mapping of specially targeted and genomic regions and the tagging of these regions with reliable PCR-based genetic markers, Crop Sci. Soc. Amer.-Amer. Soc. Hort. Sci.-Amer. Genet. Assn. Joint Plant Breeding Symp. Ser., Minneapolis. Crop. Sci. Soc. Amer., Madison, Wis p. 31–36.

    Google Scholar 

  • Reiter, R.S., Williams, J.G.K., Feldmann, K.A, and Rafalski, J.A. (1992) Global and local genome mapping in Arabidopsis thaliana by using recombinant inbred lines and random amplified polymorphic DNAs Proc. Natl. Acad. Sci. USA 89, 1477–1481.

    Article  PubMed  CAS  Google Scholar 

  • Villar, M., Lefevre, F., Bradshaw, H.D. Jr. and Cros, E. (1996) Molecular genetics of rust resistance in poplars, Genetics 143(1), 531–536.

    PubMed  CAS  Google Scholar 

  • Walbot, V. (1988) Preparation of DNA single rice seedlings, Rice Genet. 5, 149–151.

    Google Scholar 

  • Waugh, R., and Powell, W. (1992) Using RAPD markers for Crop Improvement, Tibtech 10, 186–191.

    Article  CAS  Google Scholar 

  • Williams, J.G.K., Kubelik, A R., Kenneth, J.L., Rafalski, J.A and Tinger, S. V. (1990) DNA Polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research, 18(229), 6531–6535.

    Article  PubMed  CAS  Google Scholar 

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© 1998 Springer Science+Business Media Dordrecht

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KÖrÜkmez, N., Zehİr, A., GÖZÜkirmizi, N. (1998). Identification of Genetic Polymorphism of Poplar (Populus sp.) by Dna Marker Technology. In: Tsekos, I., Moustakas, M. (eds) Progress in Botanical Research. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5274-7_120

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  • DOI: https://doi.org/10.1007/978-94-011-5274-7_120

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-6219-0

  • Online ISBN: 978-94-011-5274-7

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